The Library

Phosphate acquisition components of the Myxococcus xanthus Pho regulon are regulated by both phosphate availability and development

Tools

Whitworth, David E., Holmes, Antony B., Irvine, Alistair G., Hodgson, D. A. (David A.) and Scanlan, David J. . (2008) Phosphate acquisition components of the Myxococcus xanthus Pho regulon are regulated by both phosphate availability and development. Journal of Bacteriology, Volume 190 (Number 6). pp. 1997-2003. ISSN 0021-9193

Full text not available from this repository.

Abstract

In many organisms, phosphatase expression and phosphate (P) uptake are coordinately regulated by the Pho regulon. In Myxococcus xanthus P limitation initiates multicellular development, a process associated with changes in phosphatase expression. We sought here to characterize the link between P acquisition and development in this bacterium, an organism capable of preying upon other microorganisms as a sole nutrient source. M. xanthus seems to possess no significant internal P stores, as reducing the P concentration to less than 10 mu M retarded growth within one doubling time. Pyrophosphate, polyphosphate, and glyceraldehyde-3-phosphate could support growth as sole P sources, although many other P-containing biomolecules could not (including nucleic acids and phospholipids). Several Pho regulon promoters were found to be highly active during vegetative growth, and P limitation specifically induced pstSCAB, ACPA1, and pho3 promoter activity and repressed pit expression. Enhanced pstSCAB and pho3 promoter activities in a phoP4 mutant (in the presence of high and low concentrations of P) suggested that PhoP4 acts as a repressor of these genes. However, in a phoP4 background, the activities of pstSCAB remained P regulated, suggesting that there is additional regulation by a P-sensitive factor. Initiation of multicellular development caused immediate downregulation of Pho regulon genes and caused pstSCAB and pho3 promoter activities to become P insensitive. Hence, P acquisition components of the M. xanthus Pho regulon are regulated by both P availability and development, with developmental down-regulation overriding up-regulation by P limitation. These observations suggest that when development is initiated, subsequent changes in P availability become irrelevant to the population, which presumably has sufficient intrinsic P to ensure completion of the developmental program.

Item Type: Journal Article
Subjects: Q Science > QD Chemistry
Q Science > QR Microbiology
Divisions: Faculty of Science > Life Sciences (2010- )
Faculty of Science > Molecular Organisation and Assembly in Cells (MOAC)
Library of Congress Subject Headings (LCSH): Phosphates, Myxococcus xanthus
Journal or Publication Title: Journal of Bacteriology
Publisher: American Society for Microbiology
ISSN: 0021-9193
Official Date: March 2008
Volume: Volume 190
Number: Number 6
Number of Pages: 7
Page Range: pp. 1997-2003
Identification Number: 10.1128/JB.01781-07
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access
Funder: Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Engineering and Physical Sciences Research Council (EPSRC)
Grant number: BBD0039891 (BBSRC)
References:

1. Altschul, S. F., T. L. Madden, A. A. Scha¨ffer, J. Zhang, Z. Zhang, W. Miller,
and D. J. Lipman. 1997. Gapped BLAST and PSI-BLAST: a new generation
of protein database search programs. Nucleic Acids Res. 25:3389–3402.
2. Bretscher, A. P., and D. Kaiser. 1978. Nutrition of Myxococcus xanthus, a
fruiting myxobacterium. J. Bacteriol. 133:763–768.
3. Carrero-Le´rida, J., A. Moraleda-Mun˜oz, R. Garcı´a-Herna´ndez, J. Pe´rez, and
J. Mun˜oz-Dorado. 2005. PhoR1-PhoP1, a third two-component system of the
family PhoRP from Myxococcus xanthus: role in development. J. Bacteriol.
187:4976–4983.
4. Diodati, M. E., R. E. Gill, L. Plamann, and M. Singer. 2007. Initiation and
early developmental events, p. 43–76. In D. E. Whitworth (ed.), Myxobacteria:
multicellularity and differentiation. ASM Press, Washington, DC.
5. Higgs, P. I., and J. P. Merlie, Jr. 2007. Myxococcus xanthus: cultivation,
motility and development, p. 465–478. In D. E. Whitworth (ed.), Myxobacteria:
multicellularity and differentiation. ASM Press, Washington, DC.
6. Hodgson, D. A. 1993. Light-induced carotenogenesis in Myxococcus xanthus:
genetic analysis of the carR region. Mol. Microbiol. 7:471–488.
7. Hulett, F. M., J. Lee, L. Shi, G. Sun, R. Chesnut, E. Sharkova, M. F. Duggan,
and N. Kapp. 1994. Sequential action of two-component genetic switches
regulates the PHO regulon in Bacillus subtilis. J. Bacteriol. 176:1348–1358.
8. Hulett, F. M. 1996. The signal-transduction network for Pho regulation in
Bacillus subtilis. Mol. Microbiol. 19:933–939.
9. Itaya, K., and M. Ui. 1966. A new micromethod for the colorimetric determination
of inorganic phosphate. Clin. Chim. Acta 14:361–366.
10. Kaiser, D. 1979. Social gliding is correlated with the presence of pili in
Myxococcus xanthus. Proc. Natl. Acad. Sci. USA 76:5952–5956.
11. Liu, W., and F. M. Hulett. 1998. Comparison of PhoP binding to the tuaA
promoter with PhoP binding to other Pho-regulon promoters establishes a
Bacillus subtilis Pho core binding site. Microbiology 144:1443–1450.
12. Makino, K., M. Amemura, S.-K. Kim, A. Nakata, and H. Shinagawa. 1994.
Mechanism of transcriptional activation of the phosphate regulon in Escherichia
coli, p. 5–12. In A. Torriani-Gorini, E. Yagil, and S. Silver (ed.),
Phosphate in microorganisms: cellular and molecular biology. ASM Press,
Washington, DC.
13. Manoil, C., and D. Kaiser. 1980. Guanosine pentaphosphate and guanosine
tetraphosphate accumulation and induction of Myxococcus xanthus fruiting
body development. J. Bacteriol. 141:305–315.
14. Moraleda-Mun˜oz, A., J. Carrero-Le´rida, A. L. Extremera, J. M. Arias, and
J. Mun˜oz-Dorado. 2001. Glycerol-3-phosphate inhibits swarming and aggregation
of Myxococcus xanthus. J. Bacteriol. 183:6135–6139.
15. Moraleda-Mun˜oz, A., J. Carrero-Le´rida, J. Pe´rez, and J. Mun˜oz-Dorado. 2003.
Role of two novel two-component regulatory systems in development and phosphatase
expression in Myxococcus xanthus. J. Bacteriol. 185:1376–1383.
16. Pham, V. D., C. W. Shebelut, M. E. Diodati, C. T. Bull, and M. Singer. 2005.
Mutations affecting predation ability of the soil bacterium Myxococcus
xanthus. Microbiology 151:1865–1874.
17. Pham, V. D., C. W. Shebelut, I. R. Jose, D. A. Hodgson, D. E. Whitworth, and
M. Singer. 2006. The response regulator PhoP4 is required for late developmental
events in Myxococcus xanthus. Microbiology 152:1609–1620.
18. Qi, Y., Y. Kobayashi, and F. M. Hulett. 1997. The pst operon of Bacillus subtilis
has a phosphate-regulated promoter and is involved in phosphate transport but
not in regulation of the Pho regulon. J. Bacteriol. 179:2534–2539.
19. Scanlan, D. J., S. A. Bloye, N. H. Mann, D. A. Hodgson, and N. G. Carr. 1990.
Construction of lacZ promoter probe vectors for use in the cyanobacterium
Synechococcus R2 PCC7942: application to the identification of CO2 regulated
promoters. Gene 90:43–49.
20. Sola-Landa, A., R. S. Moura, and J. F. Martin. 2003. The two-component
PhoR-PhoP system controls both primary metabolism and secondary metabolite
biosynthesis in Streptomyces lividans. Proc. Natl. Acad. Sci. USA 100:
6133–6138.
21. Su, Z., V. Olman, and Y. Xu. 2007. Computational prediction of Pho regulons
in cyanobacteria. BMC Genomics 8:156–167.
22. Wanner, B. L. 1993. Gene regulation by phosphate in enteric bacteria.
J. Cell. Biochem. 51:47–54.
23. Weinberg, R. A., and D. R. Zusman. 1990. Alkaline, acid, and neutral phosphatase
activities are induced during development in Myxococcus xanthus. J.
Bacteriol. 172:2294–2302.
24. Zhang, H., N. N. Rao, T. Shiba, and A. Kornberg. 2005. Inorganic polyphosphate
in the social life of Myxococcus xanthus: motility, development, and
predation. Proc. Natl. Acad. Sci. USA 102:13416–13420.
URI: http://wrap.warwick.ac.uk/id/eprint/30419

Data sourced from Thomson Reuters' Web of Knowledge

Request changes or add full text files to a record

Actions (login required)

View Item View Item