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Transforming growth factor-beta 1-antisense modulates the expression of hepatocyte growth factor/scatter factor in keloid fibroblast cell culture
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Naim, R., Naumann, A., Barnes, J., Sauter, A., Hormann, K., Merkel, D., Aust, W., Braun, T. and Bloching, M. (2008) Transforming growth factor-beta 1-antisense modulates the expression of hepatocyte growth factor/scatter factor in keloid fibroblast cell culture. Aesthetic Plastic Surgery, Vol.32 (No.2). pp. 346-352. doi:10.1007/s00266-007-9078-6 ISSN 0364-216X.
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Official URL: http://dx.doi.org/10.1007/s00266-007-9078-6
Abstract
Abnormal wound healing processes can result in hypertrophic scars and keloids. Transforming growth factor-beta 1 (TGF-beta 1) and hepatocyte growth factor/scatter factor (HGF/SF) are biphasic growth factor cytokines in physiologic and pathophysiologic conditions. Findings have shown TGF-beta 1 to be pivotal in the formation of keloid tissue. Therefore, neutralizing antibodies may allow wound healing without keloid formation. As reported, TGF-beta 1 is antagonized by HGF/SF. Some authors have reported that exogenous administration of HGF/SF prevented scar formation. Hence, this study targeted TGF-beta 1 and determined the levels of HGF/SF in fibroblast cell culture. Keloid tissue was taken from seven patients. Another seven patients with mature nonhypertrophic scar served as controls. All tissues were cultured, and fibroblast cultures were used for further experiments. The TGF-beta 1 antisense was administered at 3 and 6 mu mol/ml, and HGF/SF levels were determined after 16, 24, and 48 h of incubation. The levels of HGF/SF showed significant differences after incubation with antisense oligonucleotides. The increasing antisense levels resulted in increased HGF/SF levels (up to 87.66 pg/ml after 48 h of incubation). In conclusion, targeting TGF-beta 1 resulted in significantly increased levels of HGF/SF. The clinical relevance could include the use of locally administered HGF/SF in protein or gene form to minimize formation of keloids. Nevertheless, wound healing is the result of many interacting cytokines, so neutralizing or targeting one protein could result in no significant effect.
Item Type: | Journal Article | ||||
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Subjects: | R Medicine > RD Surgery | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School | ||||
Journal or Publication Title: | Aesthetic Plastic Surgery | ||||
Publisher: | Springer | ||||
ISSN: | 0364-216X | ||||
Official Date: | March 2008 | ||||
Dates: |
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Volume: | Vol.32 | ||||
Number: | No.2 | ||||
Number of Pages: | 7 | ||||
Page Range: | pp. 346-352 | ||||
DOI: | 10.1007/s00266-007-9078-6 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
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