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Mining microbial genomes for new natural products and biosynthetic pathways

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Challis, Gregory L.. (2008) Mining microbial genomes for new natural products and biosynthetic pathways. Microbiology, Vol.154 (No.6). pp. 1555-1569. ISSN 1350-0872

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Official URL: http://dx.doi.org/10.1099/mic.0.2008/018523-0

Abstract

Analyses of microbial genome sequences have revealed numerous examples of ‘cryptic’ or ‘orphan’ biosynthetic gene clusters, with the potential to direct the production of novel, structurally complex natural products. This article summarizes the various methods that have been developed for discovering the products of cryptic biosynthetic gene clusters in microbes and gives an account of my group's discovery of the products of two such gene clusters in the model actinomycete Streptomyces coelicolor M145. These discoveries hint at new mechanisms, roles and specificities for natural product biosynthetic enzymes. Our efforts to elucidate these are described. The identification of new secondary metabolites of S. coelicolor raises the question: what is their biological function? Progress towards answering this question is also summarized.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology
Divisions:	Faculty of Science > Chemistry
Library of Congress Subject Headings (LCSH):	Microbial genomes, Streptomyces aureofaciens
Journal or Publication Title:	Microbiology
Publisher:	Society for General Microbiology
ISSN:	1350-0872
Date:	2008
Volume:	Vol.154
Number:	No.6
Page Range:	pp. 1555-1569
Identification Number:	10.1099/mic.0.2008/018523-0
Status:	Peer Reviewed
Access rights to Published version:	Open Access
Funder:	Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Wellcome Trust (London, England), European Union (EU), Marie Curie Cancer Care
Grant number:	Grant ref. 053086 (Wellcome), EGH16081 (BBSRC), B16610 (BBSRC), Contract no. 005224 (EU), Contract no. MEIF-CT-2003-501686 (Marie Curie)
Version or Related Resource:	SGM Special Lecture, Fleming Prize Lecture 2007. Delivered at the 160th meeting of the SGM, 27 March 2007.
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URI:	http://wrap.warwick.ac.uk/id/eprint/305