Richardson, Magnus J. E. and Silberberg, Gilad. (2008) Measurement and analysis of postsynaptic potentials using a novel voltage-deconvolution method. Journal of Neurophysiology, Vol.99 (No.2). pp. 1020-1031. ISSN 0022-3077

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Abstract

Accurate measurement of postsynaptic potential amplitudes is a central requirement for the quantification of synaptic strength, dynamics of short-term and long-term plasticity, and vesicle-release statistics. However, the intracellular voltage is a filtered version of the underlying synaptic signal and so a method of accounting for the distortion caused by overlapping postsynaptic potentials must be used. Here a voltage-deconvolution technique is demonstrated that defilters the entire voltage trace to reveal an underlying signal of well-separated synaptic events. These isolated events can be cropped out and reconvolved to yield a set of isolated postsynaptic potentials from which voltage amplitudes may be measured directly-greatly simplifying this common task. The method also has the significant advantage of providing a higher temporal resolution of the dynamics of the underlying synaptic signal. The versatility of the method is demonstrated by a variety of experimental examples, including excitatory and inhibitory connections to neurons with passive membranes and those with activated voltage-gated currents. The deconvolved current-clamp voltage has many features in common with voltage-clamp current measurements. These similarities are analyzed using cable theory and a multicompartment cell reconstruction, as well as direct comparison to voltage-clamp experiments.

Item Type:	Journal Article
Subjects:	R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry Q Science > QP Physiology
Divisions:	Faculty of Science > Centre for Systems Biology
Library of Congress Subject Headings (LCSH):	Neural transmission, Synapses, Synaptic vesicles, Voltage-clamp techniques (Electrophysiology)
Journal or Publication Title:	Journal of Neurophysiology
Publisher:	American Physiological Society
ISSN:	0022-3077
Date:	February 2008
Volume:	Vol.99
Number:	No.2
Number of Pages:	12
Page Range:	pp. 1020-1031
Identification Number:	10.1152/jn.00942.2007
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Human Frontier Science Program (Strasbourg, France) (HFSP), Research Councils UK
URI:	http://wrap.warwick.ac.uk/id/eprint/30558

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