Measurement and analysis of postsynaptic potentials using a novel voltage-deconvolution method
Richardson, Magnus J. E. and Silberberg, Gilad. (2008) Measurement and analysis of postsynaptic potentials using a novel voltage-deconvolution method. Journal of Neurophysiology, Vol.99 (No.2). pp. 1020-1031. ISSN 0022-3077Full text not available from this repository.
Official URL: http://dx.doi.org/10.1152/jn.00942.2007
Accurate measurement of postsynaptic potential amplitudes is a central requirement for the quantification of synaptic strength, dynamics of short-term and long-term plasticity, and vesicle-release statistics. However, the intracellular voltage is a filtered version of the underlying synaptic signal and so a method of accounting for the distortion caused by overlapping postsynaptic potentials must be used. Here a voltage-deconvolution technique is demonstrated that defilters the entire voltage trace to reveal an underlying signal of well-separated synaptic events. These isolated events can be cropped out and reconvolved to yield a set of isolated postsynaptic potentials from which voltage amplitudes may be measured directly-greatly simplifying this common task. The method also has the significant advantage of providing a higher temporal resolution of the dynamics of the underlying synaptic signal. The versatility of the method is demonstrated by a variety of experimental examples, including excitatory and inhibitory connections to neurons with passive membranes and those with activated voltage-gated currents. The deconvolved current-clamp voltage has many features in common with voltage-clamp current measurements. These similarities are analyzed using cable theory and a multicompartment cell reconstruction, as well as direct comparison to voltage-clamp experiments.
|Item Type:||Journal Article|
|Subjects:||R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Q Science > QP Physiology
|Divisions:||Faculty of Science > Centre for Systems Biology|
|Library of Congress Subject Headings (LCSH):||Neural transmission, Synapses, Synaptic vesicles, Voltage-clamp techniques (Electrophysiology)|
|Journal or Publication Title:||Journal of Neurophysiology|
|Publisher:||American Physiological Society|
|Number of Pages:||12|
|Page Range:||pp. 1020-1031|
|Access rights to Published version:||Restricted or Subscription Access|
|Funder:||Human Frontier Science Program (Strasbourg, France) (HFSP), Research Councils UK|
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