Cunliffe, Michael, Whiteley, Andrew S., Newbold, Lindsay, Oliver, Anna, Schäfer, Hendrik and Murrell, J. C. (J. Colin). (2009) Comparison of bacterioneuston and bacterioplankton dynamics during a phytoplankton bloom in a fjord mesocosm. Applied and Environmental Microbiology, Vol.75 (No.22). pp. 7173-7181. ISSN 0099-2240

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Abstract

The bacterioneuston is the community of Bacteria present in surface microlayers, the thin surface film that forms the interface between aquatic environments and the atmosphere. In this study we compared bacterial cell abundance and bacterial community structure of the bacterioneuston and the bacterioplankton (from the subsurface water column) during a phytoplankton bloom mesocosm experiment. Bacterial cell abundance, determined by flow cytometry, followed a typical bacterioplankton response to a phytoplankton bloom, with Synechococcus and high nucleic acid (HNA) bacterial cell numbers initially falling, probably due to selective protist grazing. Subsequently HNA and low nucleic acid (LNA) bacterial cells increased in abundance but Synechococcus did not. There was no significant difference between bacterioneuston and bacterioplankton cell abundances during the experiment. Conversely, distinct and consistent differences between the bacterioneuston and the bacterioplankton community structure were observed. This was monitored simultaneously by Bacteria 16S rRNA gene terminal restriction fragment length polymorphism (T-RFLP) and denaturing gradient gel electrophoresis (DGGE). The conserved patterns of community structure observed in all of the mesocosms indicate that the bacterioneuston is distinctive and non-random.

Item Type:	Journal Article
Subjects:	Q Science > QK Botany Q Science > QR Microbiology
Divisions:	Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010) Faculty of Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)
Library of Congress Subject Headings (LCSH):	Phytoplankton, Water -- Microbiology, Plankton blooms, Marine bacteria
Journal or Publication Title:	Applied and Environmental Microbiology
Publisher:	American Society for Microbiology
ISSN:	0099-2240
Date:	November 2009
Volume:	Vol.75
Number:	No.22
Page Range:	pp. 7173-7181
Identification Number:	10.1128/AEM.01374-09
Status:	Peer Reviewed
Access rights to Published version:	Open Access
Funder:	Natural Environment Research Council (Great Britain) (NERC)
Grant number:	NE/E011446/1 (NERC)
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URI:	http://wrap.warwick.ac.uk/id/eprint/3057

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