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Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds
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Castilho, Priscila V., Goto, Leandro S., Roberts, L. M. (Lynne M.) and Araujo, Ana Paula U. (2008) Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds. FEBS Journal, Vol.275 (No.5). pp. 948-959. doi:10.1111/j.1742-4658.2008.06258.x ISSN 1742-464x.
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Official URL: http://dx.doi.org/10.1111/j.1742-4658.2008.06258.x
Abstract
Abrus pulchellus seeds contain at least seven closely related and highly toxic type 2 ribosome-inactivating pulchellins, each consisting of a toxic A-chain linked to a sugar binding B-chain. In the present study, four pulchellin isoforms (termed P I, P II, P III and P IV) were isolated by affinity, ion exchange and chromatofocusing chromatographies, and investigated with respect to toxicity and sugar binding specificity. Half maximal inhibitory concentration and median lethal dose values indicate that P I and P II have similar toxicities and that both are more toxic to cultured HeLa cells and mice than P III and P IV. Interestingly, the secondary structural characteristics and sugar binding properties of the respective pairs of isoforms correlate well with the two toxicity levels, in that P I/P II and P III/P IV form two specific subgroups. From the deduced amino acids sequences of the four isoforms, it is clear that the highest similarity within each subgroup is found to occur within domain 2 of the B-chains, suggesting that the disparity in toxicity levels might be attributed to subtle differences in B-chain-mediated cell surface interactions that precede and determine toxin uptake pathways.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QD Chemistry | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Journal or Publication Title: | FEBS Journal | ||||
Publisher: | Blackwell | ||||
ISSN: | 1742-464x | ||||
Official Date: | March 2008 | ||||
Dates: |
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Volume: | Vol.275 | ||||
Number: | No.5 | ||||
Number of Pages: | 12 | ||||
Page Range: | pp. 948-959 | ||||
DOI: | 10.1111/j.1742-4658.2008.06258.x | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
Data sourced from Thomson Reuters' Web of Knowledge
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