Lack of a vacuolar sorting receptor leads to non-specific missorting of soluble vacuolar proteins in Arabidopsis seeds
Craddock, Christian P., Hunter, Paul R., Szakacs, Erika, Hinz, Giselbert, Robinson, David G. and Frigerio, Lorenzo. (2008) Lack of a vacuolar sorting receptor leads to non-specific missorting of soluble vacuolar proteins in Arabidopsis seeds. Traffic, Vol.9 (No.3). pp. 408-416. ISSN 1398-9219
WRAP_Frigerio_9870459-lf-230413-craddock_et_al_revised.pdf - Accepted Version
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Official URL: http://dx.doi.org/10.1111/j.1600-0854.2007.00693.x...
The plant vacuolar sorting receptor (VSR) binds proteins carrying vacuolar sorting signals (VSS) of the 'sequence-specific' type (ssVSS) but not the C-terminal, hydrophobic sorting signals (ctVSS). Seeds of Arabidopsis mutants lacking the major VSR isoform, AtVSR1, secrete a proportion of the proteins destined to storage vacuoles. The sorting signals for these proteins are not well defined, but they do not seem to be of the ssVSS type. Here, we tested whether absence of VSR1 in seeds leads to secretion of reporter proteins carrying ssVSS but not ctVSS. Our results show that reporters carrying either ssVSS or ctVSS are equally secreted in the absence of VSR1. We discuss our findings in relation to the current model for vacuolar sorting.
|Item Type:||Journal Article|
|Subjects:||Q Science > QH Natural history > QH301 Biology|
|Divisions:||Faculty of Science > Life Sciences (2010- )|
|Journal or Publication Title:||Traffic|
|Publisher:||Wiley-Blackwell Publishing Ltd.|
|Number of Pages:||9|
|Page Range:||pp. 408-416|
|Access rights to Published version:||Restricted or Subscription Access|
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