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Lack of a vacuolar sorting receptor leads to non-specific missorting of soluble vacuolar proteins in Arabidopsis seeds

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Craddock, Christian P., Hunter, Paul R., Szakacs, Erika, Hinz, Giselbert, Robinson, David G. and Frigerio, Lorenzo. (2008) Lack of a vacuolar sorting receptor leads to non-specific missorting of soluble vacuolar proteins in Arabidopsis seeds. Traffic, Vol.9 (No.3). pp. 408-416. ISSN 1398-9219

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Official URL: http://dx.doi.org/10.1111/j.1600-0854.2007.00693.x...

Abstract

The plant vacuolar sorting receptor (VSR) binds proteins carrying vacuolar sorting signals (VSS) of the 'sequence-specific' type (ssVSS) but not the C-terminal, hydrophobic sorting signals (ctVSS). Seeds of Arabidopsis mutants lacking the major VSR isoform, AtVSR1, secrete a proportion of the proteins destined to storage vacuoles. The sorting signals for these proteins are not well defined, but they do not seem to be of the ssVSS type. Here, we tested whether absence of VSR1 in seeds leads to secretion of reporter proteins carrying ssVSS but not ctVSS. Our results show that reporters carrying either ssVSS or ctVSS are equally secreted in the absence of VSR1. We discuss our findings in relation to the current model for vacuolar sorting.

Item Type:	Journal Article
Subjects:	Q Science > QH Natural history > QH301 Biology
Divisions:	Faculty of Science > Life Sciences (2010- )
Journal or Publication Title:	Traffic
Publisher:	Wiley-Blackwell Publishing Ltd.
ISSN:	1398-9219
Date:	March 2008
Volume:	Vol.9
Number:	No.3
Number of Pages:	9
Page Range:	pp. 408-416
Identification Number:	10.1111/j.1600-0854.2007.00693.x/abstract
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
URI:	http://wrap.warwick.ac.uk/id/eprint/30648