Microbial diversity in deep sediments of the Benguela Upwelling System
Schaefer, Hendrik, Ferdelman, Timothy G., Fossing, Henrik and Muyzer, Gerard. (2007) Microbial diversity in deep sediments of the Benguela Upwelling System. Aquatic Microbial Ecology, Vol.50 (No.1). pp. 1-9. ISSN 0948-3055Full text not available from this repository.
Official URL: http://dx.doi.org/10.3354/ame01164
The microbial diversity of sulfate-reducing and methanogenic subsurface sediments of the Benguela Upwelling System was analyzed using 16S rRNA gene-directed molecular methods. Communities mediating anaerobic oxidation of methane (AOM) in the sulfate-methane transition zone (SMTZ) were also targeted. Denaturing gradient gel electrophoresis (DGGE) of DNA extracted from sediment samples showed the presence of complex bacterial communities in the sediment zones above and below the SMTZ, whereas only 3 DGGE-defined populations were detected in the SMTZ. Sequencing of excised DNA fragments revealed the presence of bacterial populations related to sulfate-reducing bacteria (SRB) and those distantly related to the Haloanaerobium phylum. One of the populations detected at the SMTZ was closely related to SRB and to bacterial populations detected at mud volcanoes in the Black Sea. Comparative analysis of sequences from a clone library made with genomic DNA from the SMTZ and primers specific for archaeal 16S rRNA genes revealed members of 5 different lineages, including unusual crenarchaeal ribosomal RNA sequences. None of the archaeal sequences, however, was related to groups previously implicated in anaerobic methane oxidation.
|Item Type:||Journal Article|
|Subjects:||Q Science > QH Natural history > QH301 Biology
Q Science > QR Microbiology
|Divisions:||Faculty of Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)|
|Journal or Publication Title:||Aquatic Microbial Ecology|
|Date:||12 December 2007|
|Number of Pages:||9|
|Page Range:||pp. 1-9|
|Access rights to Published version:||Restricted or Subscription Access|
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