Chen, Y. (Yin), Dumont, Marc G., McNamara, Niall P., Chamberlain, P. M. (Paul M.), Bodrossy, Levente, Stralis-Pavese, Nancy and Murrell, J. C. (J. Colin). (2008) Diversity of the active methanotrophic community in acidic peatlands as assessed by mRNA and SIP-PLFA analyses. Environmental Microbiology, Vol.10 (No.2). pp. 446-459. ISSN 1462-2912

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Abstract

The active methanotroph community was investigated for the first time in heather (Calluna)-covered moorlands and Sphagnum/Eriophorum-covered UK peatlands. Direct extraction of mRNA from these soils facilitated detection of expression of methane monooxygenase genes, which revealed that particulate methane monooxygenase and not soluble methane monooxygenase was probably responsible for CH4 oxidation in situ, because only pmoA transcripts (encoding a subunit of particulate methane monooxygenase) were readily detectable. Differences in methanotroph community structures were observed between the Calluna-covered moorland and Sphagnum/Eriophorum-covered gully habitats. As with many other Sphagnum-covered peatlands, the Sphagnum/Eriophorum-covered gullies were dominated by Methylocystis. Methylocella and Methylocapsa-related species were also present. Methylobacter-related species were found as demonstrated by the use of a pmoA-based diagnostic microarray. In Calluna-covered moorlands, in addition to Methylocella and Methylocystis, a unique group of peat-associated type I methanotrophs (Gammaproteobacteria) and a group of uncultivated type II methanotrophs (Alphaproteobacteria) were also found. The pmoA sequences of the latter were only distantly related to Methylocapsa and also to the RA-14 group of methanotrophs, which are believed to be involved in oxidation of atmospheric concentrations of CH4. Soil samples were also labelled with (CH4)-C-13, and subsequent analysis of the C-13-labelled phospholipid fatty acids (PLFAs) showed that 16:1 omega 7, 18:1 omega 7 and 18:1 omega 9 were the major labelled PLFAs. The presence of C-13-labelled 18:1 omega 9, which was not a major PLFA of any extant methanotrophs, indicated the presence of novel methanotrophs in this peatland.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology
Divisions:	Faculty of Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH):	Methanotrophs, Peatland ecology, Messenger RNA, Stable isotope tracers
Journal or Publication Title:	Environmental Microbiology
Publisher:	Wiley-Blackwell Publishing Ltd.
ISSN:	1462-2912
Date:	February 2008
Volume:	Vol.10
Number:	No.2
Number of Pages:	14
Page Range:	pp. 446-459
Identification Number:	10.1111/j.1462-2920.2007.01466.x
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Natural Environment Research Council (Great Britain) (NERC), University of Warwick, Fonds zur Förderung der Wissenschaftlichen Forschung (Austria) (FWF), Centre for Ecology and Hydrology (Great Britain) (CEH)
Grant number:	NER/A/S/2002/00876 (NERC), P15044 (FWF), C03186 (CEH)
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URI:	http://wrap.warwick.ac.uk/id/eprint/30763

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