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Cryo-electron microscopy of coagulation Factor VIII bound to lipid nanotubes

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Parmenter, Christopher D. J., Cane, Matthew C., Zhang, Rui and Stollova-McPhie, Svetla. (2008) Cryo-electron microscopy of coagulation Factor VIII bound to lipid nanotubes. Biochemical and Biophysical Research Communications, Volume 366 (Number 2). pp. 288-293. ISSN 0006-291X

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Official URL: http://dx.doi.org/10.1016/j.bbrc.2007.11.072

Abstract

Factor VIII (FVIII) is a key protein in blood coagulation, deficiency or malfunction of which causes Haemophilia A. The sole cure for this condition is intravenous administration of FVIII, whose membrane-bound structure we have studied by Cryo-electron microscopy and image analysis. Self-assembled lipid nanotubes were optimised to bind FVIII at close to native conditions. The tubes diameter was constant at 30 nm and the lipid bilayer resolved. The FVIII molecules were well defined, forming an 8.5 nm thick outer layer, and appeared to reach the hydrophobic core of the bilayer. The two known FVIII atomic models were superimposed with the averaged 2D protein densities. The insertion of the FVIII within the membrane was evaluated, reaffirming that the membrane-binding C2 or C1-C2 domain(s) fully penetrate the outer leaflet of the lipid layer. The presented results lay the basis for new models of the FVIII overall orientation and membrane-binding mechanism. (C) 2007 Elsevier Inc. All rights reserved.

Item Type:

Journal Article

Subjects:

Q Science > QC Physics
Q Science > QD Chemistry
Q Science > QP Physiology

Divisions:

Faculty of Science > Chemistry

Library of Congress Subject Headings (LCSH):

Electron microscopy, Nanotubes, Blood coagulation factors, Membranes, Image analysis

Journal or Publication Title:

Biochemical and Biophysical Research Communications

Publisher:

Academic Press

ISSN:

0006-291X

Official Date:

8 February 2008

Dates:

Date	Event
8 February 2008	Published

Volume:

Volume 366

Number:

Number 2

Number of Pages:

Page Range:

pp. 288-293

Identification Number:

10.1016/j.bbrc.2007.11.072

Status:

Peer Reviewed

Publication Status:

Published

Access rights to Published version:

Restricted or Subscription Access

Funder:

British Heart Foundation, Wellcome Trust (London, England), Robert A. Welch Foundation

Grant number:

PG/04/070 (BHF), 055663/Z/98/Z (WT)

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