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Defining the structure of membrane-bound human blood coagulation factor Va
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Stoilova-McPhie, Svetla, Parmenter, C. D. J., Segers, K., Villoutreix, B. O. and Nicolaes, G. A. F. (2008) Defining the structure of membrane-bound human blood coagulation factor Va. Journal of Thrombosis and Haemostasis, Vol.6 (No.1). pp. 76-82. doi:DOI:10.1111/j.1538-7836.2007.02810.x ISSN 1538-7933.
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Official URL: http://dx.doi.org/10.1111/j.1538-7836.2007.02810.x
Abstract
Background: Blood coagulation factor (F) Va is the essential protein cofactor to the serine protease FXa. Factor Va stimulates the thrombin-to-prothrombin conversion by the prothrombinase complex, by at least five orders of magnitude. Factor Va binds with very high affinity to phosphatidylserine containing phospholipid membranes, which allows the visualization of its membrane-bound state by transmission electron microscopy (EM). Methods: In this paper we present an averaged three-dimensional structure of FVa molecules attached to phosphatidylserine containing lipid tubes, as determined by EM and single particle analysis. The low-resolution FVa three-dimensional structure is compared with the available atomic models for FVa. Results: The experimental data are combined with the most suitable atomic model and a membrane-bound FVaEM model is proposed that best fits the protein density defined by EM. In the FVaEM model, the C1 and C2 membrane-binding domains are juxtaposed onto the membrane surface and the model geometries indicate a deeper insertion of both C domains into the lipid bilayer than has been previously suggested. Conclusion: The present structure is a first step towards a higher-resolution experimental structure of a human FVa molecule in its membrane-bound conformation, allowing the visualization of individual domains within FVa and its association with the membrane.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QH Natural history > QH301 Biology R Medicine > RC Internal medicine |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Biological Sciences ( -2010) Faculty of Science, Engineering and Medicine > Science > Chemistry |
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Library of Congress Subject Headings (LCSH): | Blood coagulation factors, Electron microscopy, Membranes (Biology), Molecules -- Models, Particles -- Analysis | ||||
Journal or Publication Title: | Journal of Thrombosis and Haemostasis | ||||
Publisher: | Wiley-Blackwell Publishing Ltd. | ||||
ISSN: | 1538-7933 | ||||
Official Date: | January 2008 | ||||
Dates: |
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Volume: | Vol.6 | ||||
Number: | No.1 | ||||
Number of Pages: | 7 | ||||
Page Range: | pp. 76-82 | ||||
DOI: | DOI:10.1111/j.1538-7836.2007.02810.x | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Funder: | British Heart Foundation, Wellcome Trust (London, England), University of Warwick | ||||
Grant number: | PG/04/070 (BHF), 055663/Z/98/Z (WT) |
Data sourced from Thomson Reuters' Web of Knowledge
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