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Overexpression of a plant reticulon remodels the lumen of the cortical endoplasmic reticulum but does not perturb protein transport

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Tolley, Nicholas, Sparkes, Imogen, Hunter, P. R. (Paul R.), Craddock, Christian P., Nuttall, J. (James), Roberts, L. M. (Lynne M.), Hawes, C. R., Pedrazzini, Emanuela and Frigerio, Lorenzo. (2008) Overexpression of a plant reticulon remodels the lumen of the cortical endoplasmic reticulum but does not perturb protein transport. Traffic, Vol.9 (No.1). pp. 94-102. ISSN 1398-9219

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Official URL: http://dx.doi.org/10.1111/j.1600-0854.2007.00670.x

Abstract

We have cloned a member of the reticulon (RTN) family of Arabidopsis thaliana (RTNLB13). When fused to yellow fluorescent protein (YFP) and expressed in tobacco leaf epidermal cells, RTNLB13 is localized in the endoplasmic reticulum (ER). Coexpression of a soluble ER luminal marker reveals that YFP-tagged, myc-tagged or untagged RTNLB13 induces severe morphological changes to the lumen of the ER. We show, using fluorescence recovery after photobleaching (FRAP) analysis, that RTNLB13 overexpression greatly reduces diffusion of soluble proteins within the ER lumen, possibly by introducing constrictions into the membrane. In spite of this severe phenotype, Golgi shape, number and dynamics appear unperturbed and secretion of a reporter protein remains unaffected.

Item Type:	Journal Article
Subjects:	Q Science > QH Natural history > QH301 Biology Q Science > QK Botany
Divisions:	Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Library of Congress Subject Headings (LCSH):	Arabidopsis thaliana , Endoplasmic reticulum, Golgi apparatus, Plant organelles, Plant proteins -- Physiological transport, Secretion
Journal or Publication Title:	Traffic
Publisher:	Wiley-Blackwell Publishing Ltd.
ISSN:	1398-9219
Date:	January 2008
Volume:	Vol.9
Number:	No.1
Number of Pages:	9
Page Range:	pp. 94-102
Identification Number:	10.1111/j.1600-0854.2007.00670.x
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Oxford Brookes University
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URI:	http://wrap.warwick.ac.uk/id/eprint/30842