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Mutational analysis of the gene start sequences of pneumonia virus of mice

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Dibben, Oliver and Easton, A. J. (Andrew J.). (2007) Mutational analysis of the gene start sequences of pneumonia virus of mice. Virus Research, Vol.130 (No.1-2). pp. 303-309. ISSN 0168-1702

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Official URL: http://dx.doi.org/10.1016/j.virusres.2007.06.009

Abstract

The transcriptional start sequence of pneumonia virus of mice is more variable than that of the other pneumoviruses, with five different nine-base gene start (GS) sequences found in the PVM genome. The sequence requirements of the PVM gene start signal, and the efficiency of transcriptional initiation of the different virus genes, was investigated using a reverse genetics approach with a minigenome construct containing two reporter genes. A series of GS mutants were created, where each of the nine bases of the gene start consensus sequence of a reporter gene was changed to every other possible base, and the resulting effect on initiation of transcription was assayed. Nucleotide positions 1, 2 and 7 were found to be most sensitive to mutation whilst positions 4, 5 and 9 were relatively insensitive. The L gene GS sequence was found to have only 20% of the activity of the consensus sequence whilst the published M2 gene start sequence was found to be non-functional. A minigenome construct in which the two reporter genes were separated by the F-M2 gene junction of PVM was used to confirm the presence of two alternative, functional, GS sequences that could both drive the transcription of the PVM M2 gene.

Item Type:

Journal Article

Subjects:

Q Science > QR Microbiology > QR355 Virology

Divisions:

Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)

Library of Congress Subject Headings (LCSH):

Viral pneumonia -- Genetic aspects, Mice -- Virus diseases -- Genetic aspects, Paramyxoviruses -- Genetics

Journal or Publication Title:

Virus Research

Publisher:

Elsevier Science BV

ISSN:

0168-1702

Official Date:

December 2007

Dates:

Date	Event
December 2007	Published

Volume:

Vol.130

Number:

No.1-2

Number of Pages:

Page Range:

pp. 303-309

Identification Number:

10.1016/j.virusres.2007.06.009

Status:

Peer Reviewed

Publication Status:

Published

Access rights to Published version:

Open Access

Funder:

Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC)

References:

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