Dibben, Oliver and Easton, A. J. (Andrew J.). (2007) Mutational analysis of the gene start sequences of pneumonia virus of mice. Virus Research, Vol.130 (No.1-2). pp. 303-309. ISSN 0168-1702

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Abstract

The transcriptional start sequence of pneumonia virus of mice is more variable than that of the other pneumoviruses, with five different nine-base gene start (GS) sequences found in the PVM genome. The sequence requirements of the PVM gene start signal, and the efficiency of transcriptional initiation of the different virus genes, was investigated using a reverse genetics approach with a minigenome construct containing two reporter genes. A series of GS mutants were created, where each of the nine bases of the gene start consensus sequence of a reporter gene was changed to every other possible base, and the resulting effect on initiation of transcription was assayed. Nucleotide positions 1, 2 and 7 were found to be most sensitive to mutation whilst positions 4, 5 and 9 were relatively insensitive. The L gene GS sequence was found to have only 20% of the activity of the consensus sequence whilst the published M2 gene start sequence was found to be non-functional. A minigenome construct in which the two reporter genes were separated by the F-M2 gene junction of PVM was used to confirm the presence of two alternative, functional, GS sequences that could both drive the transcription of the PVM M2 gene.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology > QR355 Virology
Divisions:	Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Library of Congress Subject Headings (LCSH):	Viral pneumonia -- Genetic aspects, Mice -- Virus diseases -- Genetic aspects, Paramyxoviruses -- Genetics
Journal or Publication Title:	Virus Research
Publisher:	Elsevier Science BV
ISSN:	0168-1702
Date:	December 2007
Volume:	Vol.130
Number:	No.1-2
Number of Pages:	7
Page Range:	pp. 303-309
Identification Number:	10.1016/j.virusres.2007.06.009
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Open Access
Funder:	Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC)
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URI:	http://wrap.warwick.ac.uk/id/eprint/30866

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