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Light enhanced amino acid uptake by dominant bacterioplankton groups in surface waters of the Atlantic Ocean

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Mary, Isabelle, Tarran, Glen A. , Warwick, Phillip E., Terry, Matthew J. , Scanlan, David J. , Burkill, Peter H. and Zubkov, Mikhail V. . (2008) Light enhanced amino acid uptake by dominant bacterioplankton groups in surface waters of the Atlantic Ocean. FEMS Microbiology Ecology, Vol.63 (No.1). pp. 36-45. ISSN 0168-6496

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Official URL: http://dx.doi.org/10.1111/j.1574-6941.2007.00414.x

Abstract

S-35-Methionine and H-3-leucine bioassay tracer experiments were conducted on two meridional transatlantic cruises to assess whether dominant planktonic microorganisms use visible sunlight to enhance uptake of these organic molecules at ambient concentrations. The two numerically dominant groups of oceanic bacterioplankton were Prochlorococcus cyanobacteria and bacteria with low nucleic acid (LNA) content, comprising 60% SAR11-related cells. The results of flow cytometric sorting of labelled bacterioplankton cells showed that when incubated in the light, Prochlorococcus and LNA bacteria increased their uptake of amino acids on average by 50% and 23%, respectively, compared with those incubated in the dark. Amino acid uptake of Synechococcus cyanobacteria was also enhanced by visible light, but bacteria with high nucleic acid content showed no light stimulation. Additionally, differential uptake of the two amino acids by the Prochlorococcus and LNA cells was observed. The populations of these two types of cells on average completely accounted for the determined 22% light enhancement of amino acid uptake by the total bacterioplankton community, suggesting a plausible way of harnessing light energy for selectively transporting scarce nutrients that could explain the numerical dominance of these groups in situ.

Item Type:

Journal Article

Subjects:

Q Science > QH Natural history > QH301 Biology
Q Science > QK Botany
Q Science > QR Microbiology

Divisions:

Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)

Library of Congress Subject Headings (LCSH):

Marine phytoplankton -- Atlantic Ocean, Amino acids -- Physiological transport, Flow cytometry, Biological assay, Tracers (Chemistry)

Journal or Publication Title:

FEMS Microbiology Ecology

Publisher:

Wiley-Blackwell Publishing Ltd.

ISSN:

0168-6496

Official Date:

January 2008

Dates:

Date	Event
January 2008	Published

Volume:

Vol.63

Number:

No.1

Number of Pages:

Page Range:

pp. 36-45

Identifier:

10.1111/j.1574-6941.2007.00414.x

Status:

Peer Reviewed

Publication Status:

Published

Access rights to Published version:

Restricted or Subscription Access

Funder:

Natural Environment Research Council (Great Britain) (NERC), Natural Environment Research Council (Great Britain). Marine Microbial Metagenomics consortium, National Oceanography Centre (Great Britain), Plymouth Marine Laboratory (Great Britain)

Grant number:

NE/C514723/1 (NERC), NE/C50800X/1 (NERCMMM), NER/I/S/2000/01426 (NERC)

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