Influence of c-Myc and bcl-2 CO-expression on proliferation, apoptosis and survival in CHO cultures
Ifandi, Vasiliki and Al-Rubeai, Mohamed (2007) Influence of c-Myc and bcl-2 CO-expression on proliferation, apoptosis and survival in CHO cultures. In: 19th Meeting of the European-Society-for-Animal-Cell-Technology (ESACT), Harrogate, UK, 5-8 Jun 2005. Published in: Cell Technology for Cell Products : Proceedings of the 19th ESACT Meeting, Harrogate, UK, June 5-8, 2005, Volume 3 pp. 217-220.Full text not available from this repository.
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Under the constitutive control of c-Myc over-expression the CHO cultures showed an increase in growth rate and maximum cell number accompanied by a similar decrease in specific glucose consumption rate. Additionally overexpression of c-Myc appeared to induce morphological transformation and partial anchorage-independence. Although, the c-myc transfected cell line exhibited apoptosis at much lower rates than it is widely reported and associated with the over-expression of c-Myc, it was nevertheless apparent that c-Myc was responsible for the induction of higher apoptotic rates when compared with the control cell line. Hence, the anti-apoptotic gene bcl-2 was also used to transfect the c-Myc CHO cell line, in order to reduce cell death. Over-expression of both oncoproteins resulted in a cell line that exhibited higher proliferation rates and maximum cell numbers, with a decrease in apoptosis when compared to the parental cell line.
|Item Type:||Conference Item (Paper)|
|Subjects:||T Technology > TP Chemical technology
Q Science > QH Natural history > QH301 Biology
|Journal or Publication Title:||Cell Technology for Cell Products : Proceedings of the 19th ESACT Meeting, Harrogate, UK, June 5-8, 2005|
|Number of Pages:||4|
|Page Range:||pp. 217-220|
|Conference Paper Type:||Paper|
|Title of Event:||19th Meeting of the European-Society-for-Animal-Cell-Technology (ESACT)|
|Type of Event:||Conference|
|Location of Event:||Harrogate, UK|
|Date(s) of Event:||5-8 Jun 2005|
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