Ifandi, Vasiliki and Al-Rubeai, Mohamed (2007) Influence of c-Myc and bcl-2 CO-expression on proliferation, apoptosis and survival in CHO cultures. In: 19th Meeting of the European-Society-for-Animal-Cell-Technology (ESACT), Harrogate, ENGLAND, JUN 05-08, 2005. Published in: Cell Technology for Cell Products pp. 217-220.

Full text not available from this repository.

Abstract

Under the constitutive control of c-Myc over-expression the CHO cultures showed an increase in growth rate and maximum cell number accompanied by a similar decrease in specific glucose consumption rate. Additionally overexpression of c-Myc appeared to induce morphological transformation and partial anchorage-independence. Although, the c-myc transfected cell line exhibited apoptosis at much lower rates than it is widely reported and associated with the over-expression of c-Myc, it was nevertheless apparent that c-Myc was responsible for the induction of higher apoptotic rates when compared with the control cell line. Hence, the anti-apoptotic gene bcl-2 was also used to transfect the c-Myc CHO cell line, in order to reduce cell death. Over-expression of both oncoproteins resulted in a cell line that exhibited higher proliferation rates and maximum cell numbers, with a decrease in apoptosis when compared to the parental cell line.

Item Type:	Conference Item (UNSPECIFIED)
Subjects:	T Technology > TP Chemical technology Q Science > QH Natural history > QH301 Biology
Journal or Publication Title:	Cell Technology for Cell Products
Publisher:	SPRINGER
ISBN:	978-1-4020-5475-4
Editor:	Smith, R
Date:	2007
Number of Pages:	4
Page Range:	pp. 217-220
Publication Status:	Published
Title of Event:	19th Meeting of the European-Society-for-Animal-Cell-Technology (ESACT)
Location of Event:	Harrogate, ENGLAND
Date(s) of Event:	JUN 05-08, 2005
URI:	http://wrap.warwick.ac.uk/id/eprint/30987

Data sourced from Thomson Reuters' Web of Knowledge

Request changes to a record

Actions (login required)

View Item