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E-cadherin and cell adhesion : a role in architecture and function in the pancreatic islet
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Rogers, Gareth J., Hodgkin, Matthew N. and Squires, Paul E. (2007) E-cadherin and cell adhesion : a role in architecture and function in the pancreatic islet. Cellular Physiology and Biochemistry, Volume 20 (Number 6). pp. 987-994. doi:10.1159/000110459 ISSN 1015-8987.
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Official URL: http://dx.doi.org/10.1159/000110459
Abstract
Background/Aims: The efficient secretion of insulin from beta-cells requires extensive intra-islet communication. The cell surface adhesion protein epithelial (E)-cadherin (ECAD) establishes and maintains epithelial tissues such as the islets of Langerhans. In this study, the role of ECAD in regulating insulin secretion from pseudoislets was investigated. Methods: The effect of an immuno-neutralising ECAD on gross morphology, cytosolic calcium signalling, direct cell-to-cell communication and insulin secretion was assessed by fura-2 microfluorimetry, Lucifer Yellow dye injection and insulin ELISA in an insulin-secreting model system. Results: Antibody blockade of ECAD reduces glucose-evoked changes in [Ca2+](i) and insulin secretion. Neutralisation of ECAD causes a breakdown in the glucose-stimulated synchronicity of calcium oscillations between discrete regions within the pseudoislet, and the transfer of dye from an individual cell within a cell cluster is attenuated in the absence of ECAD ligation, demonstrating that gap junction communication is disrupted. The functional consequence of neutralising ECAD is a significant reduction in insulin secretion. Conclusion: Cell adhesion via ECAD has distinct roles in the regulation of intercellular communication between beta-cells within islets, with potential repercussions for insulin secretion. Copyright (C) 2007 S. Karger AG, Basel.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QP Physiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Biological Sciences ( -2010) | ||||
Journal or Publication Title: | Cellular Physiology and Biochemistry | ||||
Publisher: | Karger | ||||
ISSN: | 1015-8987 | ||||
Official Date: | 2007 | ||||
Dates: |
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Volume: | Volume 20 | ||||
Number: | Number 6 | ||||
Number of Pages: | 8 | ||||
Page Range: | pp. 987-994 | ||||
DOI: | 10.1159/000110459 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published |
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