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E-cadherin and cell adhesion: a role in architecture and function in the pancreatic islet
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Rogers, Gareth J., Hodgkin, Matthew N. and Squires, Paul E.. (2007) E-cadherin and cell adhesion: a role in architecture and function in the pancreatic islet. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 20 (6). pp. 987-994. ISSN 1015-8987
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Official URL: http://dx.doi.org/10.1159/000110459
Abstract
Background/Aims: The efficient secretion of insulin from beta-cells requires extensive intra-islet communication. The cell surface adhesion protein epithelial (E)-cadherin (ECAD) establishes and maintains epithelial tissues such as the islets of Langerhans. In this study, the role of ECAD in regulating insulin secretion from pseudoislets was investigated. Methods: The effect of an immuno-neutralising ECAD on gross morphology, cytosolic calcium signalling, direct cell-to-cell communication and insulin secretion was assessed by fura-2 microfluorimetry, Lucifer Yellow dye injection and insulin ELISA in an insulin-secreting model system. Results: Antibody blockade of ECAD reduces glucose-evoked changes in [Ca2+](i) and insulin secretion. Neutralisation of ECAD causes a breakdown in the glucose-stimulated synchronicity of calcium oscillations between discrete regions within the pseudoislet, and the transfer of dye from an individual cell within a cell cluster is attenuated in the absence of ECAD ligation, demonstrating that gap junction communication is disrupted. The functional consequence of neutralising ECAD is a significant reduction in insulin secretion. Conclusion: Cell adhesion via ECAD has distinct roles in the regulation of intercellular communication between beta-cells within islets, with potential repercussions for insulin secretion. Copyright (C) 2007 S. Karger AG, Basel.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QP Physiology |
| Journal or Publication Title: | CELLULAR PHYSIOLOGY AND BIOCHEMISTRY |
| Publisher: | KARGER |
| ISSN: | 1015-8987 |
| Date: | 2007 |
| Volume: | 20 |
| Number: | 6 |
| Number of Pages: | 8 |
| Page Range: | pp. 987-994 |
| Identification Number: | 10.1159/000110459 |
| Publication Status: | Published |
| URI: | http://wrap.warwick.ac.uk/id/eprint/31101 |
Data sourced from Thomson Reuters' Web of Knowledge
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