1. Gerth, K., Pradella, S., Perlova, O., Beyer, S. & Mu¨ ller, R. Myxobacteria: proficient
producers of novel natural products with various biological activities – past and future
biotechnological aspects with the focus on the genus Sorangium. J. Biotechnol. 106,
233–253 (2003).
2. Gerth, K., Bedorf, N., Ho¨fle, G., Irschik, H. & Reichenbach, H. Epothilons A and B:
antifungal and cytotoxic compounds from Sorangium cellulosum (Myxobacteria).
Production, physico-chemical and biological properties. J. Antibiot. (Tokyo) 49,
560–563 (1996).
3. Fischbach, M.A. & Walsh, C.T. Assembly-line enzymology for polyketide and nonribosomal
peptide antibiotics: logic, machinery, and mechanisms. Chem. Rev. 106,
3468–3496 (2006).
4. Gerth, K., Perlova, O. & Mu¨ ller, R. Sorangium cellulosum. in Multicellularity and
Differentiation Among the Myxobacteria and Their Neighbors (eds. Kaplan, H.B. &
Whitworth, D.) (ASM press, Washington DC, in the press).
5. Reichenbach, H. Order VIII. Myxococcales Tchan, Pochon and Pre´vot 1948, 398AL. in
Bergey’s Manual of Systematic Bacteriology (eds. Brenner, D.J., Krieg, N.R., Staley, J.T.
& Garrity, G.M.) 1059–1072 (Springer, New York, 2005).
6. Goldman, B.S. et al. Evolution of sensory complexity recorded in a myxobacterial
genome. Proc. Natl. Acad. Sci. USA 103, 15200–15205 (2006).
7. Pradella, S. et al. Characterisation, genome size and genetic manipulation of the
myxobacterium Sorangium cellulosum So ce56. Arch. Microbiol. 178, 484–492
(2002).
8. Gaitatzis, N., Kunze, B. & Mu¨ ller, R. Novel insights into siderophore formation in
myxobacteria. ChemBioChem 6, 365–374 (2005).
9. Tu, Q. & Ding, D.F. Detecting pathogenicity islands and anomalous gene clusters
by iterative discriminant analysis. FEMS Microbiol. Lett. 221, 269–275 (2003).
10.Brennan, M.J. & Delogu, G. The PE multigene family: a ‘molecular mantra’ for
mycobacteria. Trends Microbiol. 10, 246–249 (2002).
11.Srinivasan, B.S. et al. Functional genome annotation through phylogenomic mapping.
Nat. Biotechnol. 23, 691–698 (2005).
12.Davidson, G.S., Wylie, B.N. & Boyack, K.W. Cluster stability and the use of noise in
interpretation of clustering. Proceedings of the IEEE Symposium on Information
Visualization 2001 (INFOVIS’01), 23–30 (2007).
13.Eppinger, M., Baar, C., Raddatz, G., Huson, D.H. & Schuster, S.C. Comparative analysis
of four Campylobacterales. Nat. Rev. Microbiol. 2, 872–885 (2004).
14. Lawrence, J.G. Gene organization: selection, selfishness, and serendipity. Annu. Rev.
Microbiol. 57, 419–440 (2003).
15.Bretscher, A.P. & Kaiser, D. Nutrition of Myxococcus xanthus, a fruiting myxobacterium.
J. Bacteriol. 133, 763–768 (1978).
16.Watson, B.F. & Dworkin, M. Comparative intermediary metabolism of vegetative
cells and microcysts of Myxococcus xanthus. J. Bacteriol. 96, 1465–1473
(1968).
17. Youderian, P., Burke, N., White, D.J. & Hartzell, P.L. Identification of genes required for
adventurous gliding motility in Myxococcus xanthus with the transposable element
mariner. Mol. Microbiol. 49, 555–570 (2003).
18. Youderian, P. & Hartzell, P.L. Transposon insertions of magellan-4 that impair social
gliding motility in Myxococcus xanthus. Genetics 172, 1397–1410 (2006).
19.Brocchieri, L. & Karlin, S. Protein length in eukaryotic and prokaryotic proteomes.
Nucleic Acids Res. 33, 3390–3400 (2005).
20. Choudhuri, J.V., Schleiermacher, C., Kurtz, S. & Giegerich, R. GenAlyzer: interactive
visualization of sequence similarities between entire genomes. Bioinformatics 20,
1964–1965 (2004).
21. Inouye, S., Nariya, H. & Mun˜ oz-Dorado, J. Protein Ser/Thr kinases and phosphatases in
Myxococcus xanthus. in Multicellularity and Differentiation Among the Myxobacteria
and Their Neighbors (eds. Kaplan, H.B. & Whitworth, D.) (ASM Press, Washington D.C.,
in the press).
22.Kannan, N., Taylor, S.S., Zhai, Y., Venter, J.C. & Manning, G. Structural and functional
diversity of the microbial kinome. PLoS Biol. 5, e17 (2007).
23.Wang, L., Sun, Y.P., Chen, W.L., Li, J.H. & Zhang, C.C. Genomic analysis of protein
kinases, protein phosphatases and two-component regulatory systems of the cyanobacterium
Anabaena sp. strain PCC 7120. FEMS Microbiol. Lett. 217, 155–165
(2002).
24. Petrickova, K. & Petricek, M. Eukaryotic-type protein kinases in Streptomyces coelicolor:
variations on a common theme. Microbiology 149, 1609–1621 (2003).
25. Irschik, H. et al. Etnangien, a macrolide-polyene antibiotic from Sorangium cellulosum
that inhibits nucleic acid polymerases. J. Nat. Prod. 70, 1060–1063 (2007).
26.Wenzel, S.C. & Mu¨ ller, R. Myxobacterial natural product assembly lines: fascinating
examples of curious biochemistry. Nat. Prod. Rep. (in the press).
27. Schley, C., Altmeyer, M.O., Swart, R., Mu¨ ller, R. & Huber, C.G. Proteome analysis of
Myxococcus xanthus by off-line two-dimensional chromatographic separation using
monolithic poly-(styrene-divinylbenzene) columns combined with ion-trap tandem
mass spectrometry. J. Proteome Res. 5, 2760–2768 (2006).
28. Schmidt-Dannert, C., Umeno, D. & Arnold, F.H. Molecular breeding of carotenoid
biosynthetic pathways. Nat. Biotechnol. 18, 750–753 (2000).
29.Dickschat, J.S., Bode, H.B., Mahmud, T., Mu¨ ller, R. & Schulz, S. A novel type of
geosmin biosynthesis in myxobacteria. J. Org. Chem. 70, 5174–5182 (2005).
30. Jiang, J., He, X. & Cane, D.E. Geosmin biosynthesis: Streptomyces coelicolor germacradienol/
germacrene D synthase converts farnesyl diphosphate to geosmin. J. Am.
Chem. Soc. 128, 8128–8129 (2006).
31.Bode, H.B. & Mu¨ ller, R. Possibility of bacterial recruitment of plant genes associated
with the biosynthesis of secondary metabolites. Plant Physiol. 132, 1153–1161
(2003).
32.Ueda, K., Kim, K.M., Beppu, T. & Horinouchi, S. Overexpression of a gene cluster
encoding a chalcone synthase-like protein confers redbrown pigment production in
Streptomyces griseus. J. Antibiot. (Tokyo) 48, 638–646 (1995).
33.Gross, F. et al. Bacterial type III polyketide synthases: phylogenetic analysis and
potential for the production of novel secondary metabolites by heterologous expession
in pseudomonads. Arch. Microbiol. 185, 28–38 (2006).
34.Rosenau, F., Tommassen, J. & Jaeger, K.E. Lipase-specific foldases. ChemBioChem 5,
152–161 (2004).
35.Kroutil, W., Mang, H., Edegger, K. & Faber, K. Recent advances in the biocatalytic
reduction of ketones and oxidation of sec-alcohols. Curr. Opin. Chem. Biol. 8, 120–126
(2004).
36.Rey, M.W. et al. Complete genome sequence of the industrial bacterium Bacillus
licheniformis and comparisons with closely related Bacillus species. Genome Biol. 5,
R77 (2004).
37. Coucke, P. & Voets, J.P. The study of enzymatic cellulolysis of Sorangium composition.
Ann. Inst. Pasteur (Paris) 115, 549–560 (1968).
38.Rachid, S., Gerth, K., Kochems, I. & Mu¨ ller, R. Deciphering regulatory mechanisms for
secondary metabolite production in the myxobacterium Sorangium cellulosum So
ce56. Mol. Microbiol. 63, 1783–1796 (2007).
39. Ewing, B., Hillier, L., Wendl, M.C. & Green, P. Base-calling of automated sequencer
traces using phred. I. Accuracy assessment. Genome Res. 8, 175–185 (1998).
40.Kaiser, O. et al. Whole genome shotgun sequencing guided-by bioinformatics
pipelines – an optimized approach for an established technique. J. Biotechnol. 106,
121–133 (2003).
41.Gordon, D., Desmarais, C. & Green, P. Automated finishing with autofinish. Genome
Res. 11, 614–625 (2001).
42.Bartels, D. et al. BACCardI – a tool for the validation of genomic assemblies, assisting
genome finishing and intergenome comparison. Bioinformatics 21, 853–859 (2005).
43.Meyer, F. et al. GenDB – an open source genome annotation system for prokaryote
genomes. Nucleic Acids Res. 31, 2187–2195 (2003).
44. Altschul, S.F. et al. Gapped BLAST and PSI-BLAST: a new generation of protein
database search programs. Nucleic Acids Res. 25, 3389–3402 (1997).
45.Harris, M.A. et al. The Gene Ontology (GO) database and informatics resource. Nucleic
Acids Res. 32, D258–D261 (2004).
46.Boyle, E.I. et al. GO:TermFinder – open source software for accessing gene ontology
information and finding significantly enriched gene ontology terms associated with a
list of genes. Bioinformatics 20, 3710–3715 (2004).
47. Perlova, O., Gerth, K., Hans, A., Kaiser, O. & Mu¨ ller, R. Identification and analysis of
the chivosazol biosynthetic gene cluster from the myxobacterial model strain Sorangium
cellulosum So ce56. J. Biotechnol. 121, 174–191 (2006).
48.Kjellstrom, S. & Jensen, O.N. Phosphoric acid as a matrix additive for MALDI MS
analysis of phosphopeptides and phosphoproteins. Anal. Chem. 76, 5109–5117
(2004).
49. Pappin, D.J., Test, G.-Q., Test, M.-M., Hojrup, P. & Bleasby, A.J. Rapid identification of
proteins by peptide-mass fingerprinting. Curr. Biol. 3, 327–332 (1993).
50. Tholey, A., Reed, J. & Lehmann, W.D. Electrospray tandem mass spectrometric studies
of phosphopeptides and phosphopeptide analogues. J. Mass Spectrom. 34, 117–123
(1999).