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Effects of Angiotensin II on human endothelial cells survival signalling pathways and its angiogenic response
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Kou, Baijun, Vatish, Manu and Singer, Donald R. J. (2007) Effects of Angiotensin II on human endothelial cells survival signalling pathways and its angiogenic response. Vascular Pharmacology, Vol.47 (No.4). pp. 199-208. doi:10.1016/j.vph.2007.06.011 ISSN 1537-1891.
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Official URL: http://dx.doi.org/10.1016/j.vph.2007.06.011
Abstract
Reduced capillary density (rarefaction) is an early event of cardiovascular disease. The PI-3K-Akt pathway is a key player in anti-endothelial cells (ECs) apoptosis. VEGF is a key growth factor for angiogenesis. We investigated the effect of Angiotensin II (Ang II) on ECs survival signalling and angiogenesis in vitro. We found that Ang II had a biphasic effect on Akt phosphorylation by western blotting analysis. Low concentration Ang II caused a dose-dependent increase in Akt phosphorylation, while high concentration of Ang II led to a decrease of Akt phosphorylation. This effect was negative regulated by its type II receptor. Ang II 10(-4) M induced ECs apoptosis by its type H receptor was completely blocked by VEGF. Cell viability was increased by Ang II 10(-6) M and decreased by Ang II 10(-4) M. It was further decreased by pre-treatment with PI-3K/Akt inhibitor LY294002, but unaffected by p38-MAPK inhibitor SB202190. Ang II 10(-4) M reduced ECs' proliferation and vascular tube length, which were in part regulated by type II receptor. Our findings support a dose-dependent role of Ang II in effect on ECs survival and angiogenesis by PI-3K/Akt pathway. The anti-angiogenic effect of Ang H was mediated by its type II receptor.
Item Type: | Journal Article | ||||
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Subjects: | R Medicine > RS Pharmacy and materia medica | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School | ||||
Journal or Publication Title: | Vascular Pharmacology | ||||
Publisher: | Elsevier Inc | ||||
ISSN: | 1537-1891 | ||||
Official Date: | October 2007 | ||||
Dates: |
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Volume: | Vol.47 | ||||
Number: | No.4 | ||||
Number of Pages: | 10 | ||||
Page Range: | pp. 199-208 | ||||
DOI: | 10.1016/j.vph.2007.06.011 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
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