The role of ornithine aminotransferase in fruiting body formation of the mushroom Agaricus bisporus
Wagemaker, Matthijs J. M., Eastwood, Daniel C., Welagen, Jelle, Van der Drift, Chris, Jetten, Mike S. M., Burton, Kerry S., Van Griensven, Leo J. L. D. and Den Campa, Huub J. M. Op. (2007) The role of ornithine aminotransferase in fruiting body formation of the mushroom Agaricus bisporus. Mycological Research, Vol.111 (No.8). pp. 909-918. ISSN 0953-7562Full text not available from this repository.
Official URL: http://dx.doi.org/10.1016/j.mycres.2007.05.012
The complete oat gene and cDNA from the commercial mushroom, Agaricus bisporus, encoding ornithine aminotransferase (OAT) was characterized. The gene encodes a 466 amino acid protein and provides the first fully reported homobasidiomycete OAT protein sequence. The gene is interrupted by ten introns, and no mitochondrial targeting motif was present pointing to a cytoplasmic localization. The function of the gene was demonstrated by complementation of a Saccharomyces cerevisiae mutant unable to utilize ornithine as a sole source of nitrogen with an A. bisporus oat cDNA construct. Northern analysis of the oat gene together with the pruA gene (encoding Δ1-pyrroline-5-carboxylate dehydrogenase) showed that transcripts of both genes were lower during the first stages of fruiting body development. The higher expression of the oat gene in later stages of development, suggests the importance of ornithine metabolism for the redistribution of metabolites in the developing mushroom. Hplc analysis of all amino acids revealed that ornithine levels increased during fruiting body development whereas proline levels fell.
|Item Type:||Journal Article|
|Subjects:||Q Science > QK Botany|
|Divisions:||Faculty of Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)|
|Journal or Publication Title:||Mycological Research|
|Publisher:||Cambridge University Press|
|Official Date:||August 2007|
|Number of Pages:||10|
|Page Range:||pp. 909-918|
|Access rights to Published version:||Restricted or Subscription Access|
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