The reference genetic linkage map for the multinational Brassica rapa genome sequencing project
Choi, Su Ryun, Teakle, Graham R., Plaha, Prikshit, Kim, Jeong Hee, Allender, Charlotte J., Beynon, Elena, Piao, Zhong Yun, Soengas, Pilar, Han, Tae Ho, King, Graham J., Barker, Guy C., Hand, Paul, Lydiate, Derek J., Batley, Jacqueline, Edwards, David, Koo, Dal Hoe, Bang, Jae Wook, Park, Beom-Seok and Lim, Yong Pyo. (2007) The reference genetic linkage map for the multinational Brassica rapa genome sequencing project. Theoretical and Applied Genetics, Vol.115 (No.6). pp. 777-792. ISSN 0040-5752Full text not available from this repository.
Official URL: http://dx.doi.org/10.1007/s00122-007-0608-z
We describe the construction of a reference genetic linkage map for the Brassica A genome, which will form the backbone for anchoring sequence contigs for the Multinational Brassica rapa Genome Sequencing Project. Seventy-eight doubled haploid lines derived from anther culture of the F-1 of a cross between two diverse Chinese cabbage (B. rapa ssp. pekinensis) inbred lines, 'Chiifu-401-42' (C) and 'Kenshin-402-43' (K) were used to construct the map. The map comprises a total of 556 markers, including 278 AFLP, 235 SSR, 25 RAPD and 18 ESTP, STS and CAPS markers. Ten linkage groups were identified and designated as R1-R10 through alignment and orientation using SSR markers in common with existing B. napus reference linkage maps. The total length of the linkage map was 1,182 cM with an average interval of 2.83 cM between adjacent loci. The length of linkage groups ranged from 81 to 161 cM for R04 and R06, respectively. The use of 235 SSR markers allowed us to align the A-genome chromosomes of B. napus with those of B. rapa ssp. pekinensis. The development of this map is vital to the integration of genome sequence and genetic information and will enable the international research community to share resources and data for the improvement of B. rapa and other cultivated Brassica species.
|Item Type:||Journal Article|
S Agriculture > SB Plant culture
Q Science > QH Natural history > QH426 Genetics
|Divisions:||Faculty of Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)|
|Journal or Publication Title:||Theoretical and Applied Genetics|
|Number of Pages:||16|
|Page Range:||pp. 777-792|
|Access rights to Published version:||Restricted or Subscription Access|
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