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High-energy collision induced dissociation fragmentation pathways of peptides, probed using a multiturn tandem time-of-flight mass spectrometer "MULTUM-TOF/TOF"
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Toyoda, Michisato, Giannakopulos, Anastassios E., Colburn, Alex W. and Derrick, Peter J. (2007) High-energy collision induced dissociation fragmentation pathways of peptides, probed using a multiturn tandem time-of-flight mass spectrometer "MULTUM-TOF/TOF". Review of Scientific Instruments, Vol.78 (No.7). Article: 074101. doi:10.1063/1.2751403 ISSN 0034-6748.
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Official URL: http://dx.doi.org/10.1063/1.2751403
Abstract
A new multiturn tandem time-of-flight (TOF) mass spectrometer "MULTUM-TOF/TOF" has been designed and constructed. It consists of a matrix-assisted laser desorption/ionization ion source, a multiturn TOF mass spectrometer, a collision cell, and a quadratic-field ion mirror. The multiturn TOF mass spectrometer can overcome the problem of precursor ion selection in TOF, due to insufficient time separation between two adjacent TOF peaks, by increasing the number of cycles. As a result, the total TOF increases with the increase in resolving power. The quadratic-field ion mirror allows temporal focusing for fragment ions with different kinetic energies. Product ion spectra from monoisotopically selected precursor ions of angiotensin I, substance P, and bradykinin have been obtained. The fragment ions observed are mainly the result of high-energy collision induced dissociation. (c) 2007 American Institute of Physics.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QC Physics | ||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||
| Journal or Publication Title: | Review of Scientific Instruments | ||||
| Publisher: | American Institute of Physics | ||||
| ISSN: | 0034-6748 | ||||
| Official Date: | July 2007 | ||||
| Dates: |
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| Volume: | Vol.78 | ||||
| Number: | No.7 | ||||
| Number of Pages: | 9 | ||||
| Page Range: | Article: 074101 | ||||
| DOI: | 10.1063/1.2751403 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published |
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