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Partial characterisation of five cloned viruses differing in pathogenicity, obtained from a single isolate of pigeon paramyxovirus type 1 (PPMV-1) following passage in fowls' eggs
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Fuller, C. M., Collins, M. S., Easton, A. J. (Andrew J.) and Alexander, D. J. (2007) Partial characterisation of five cloned viruses differing in pathogenicity, obtained from a single isolate of pigeon paramyxovirus type 1 (PPMV-1) following passage in fowls' eggs. Archives of Virology, Vol.152 (No.8). pp. 1575-1582. doi:10.1007/s00705-007-0963-8
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Official URL: http://dx.doi.org/10.1007/s00705-007-0963-8
Abstract
Viruses with intracerebral pathogenicity indices (ICPIs) of 0.025, 0.55, 1.013 and 1.3. were cloned from a PPMV-1 isolate with an ICPI of 0.32 by passage in embryonated fowls' eggs. Deduced amino acid sequences of the haemagglutinin-neuraminidase (HN) and precursor fusion proteins (F0) showed them to have only a single amino acid difference: those with an ICPI value < 0.7 had proline at amino acid position 453 of the F0 protein, and those with an ICPI value > 0.7 contained a serine. The virus with an ICPI of 0.025 was further passaged, and the ICPI of non-cloned virus increased to 0.76/0.79, which was then reduced to 0.49 on cloning. The proline at residue 453 was retained, but there were two nucleotide changes in the virus of ICPI 0.49, T -> C at position 1769 in the untranslated region of the fusion gene and G -> A at position 437 of the HN gene, resulting in the amino acid change G -> R at position 116 in the HN protein.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QR Microbiology > QR355 Virology | ||||
| Divisions: | Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010) | ||||
| Journal or Publication Title: | Archives of Virology | ||||
| Publisher: | Springer Wien | ||||
| ISSN: | 0304-8608 | ||||
| Official Date: | August 2007 | ||||
| Dates: |
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| Volume: | Vol.152 | ||||
| Number: | No.8 | ||||
| Number of Pages: | 8 | ||||
| Page Range: | pp. 1575-1582 | ||||
| DOI: | 10.1007/s00705-007-0963-8 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
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