Partial characterisation of five cloned viruses differing in pathogenicity, obtained from a single isolate of pigeon paramyxovirus type 1 (PPMV-1) following passage in fowls' eggs
Fuller, C. M., Collins, M. S., Easton, A. J. (Andrew J.) and Alexander, D. J.. (2007) Partial characterisation of five cloned viruses differing in pathogenicity, obtained from a single isolate of pigeon paramyxovirus type 1 (PPMV-1) following passage in fowls' eggs. Archives of Virology, Vol.152 (No.8). pp. 1575-1582. ISSN 0304-8608Full text not available from this repository.
Official URL: http://dx.doi.org/10.1007/s00705-007-0963-8
Viruses with intracerebral pathogenicity indices (ICPIs) of 0.025, 0.55, 1.013 and 1.3. were cloned from a PPMV-1 isolate with an ICPI of 0.32 by passage in embryonated fowls' eggs. Deduced amino acid sequences of the haemagglutinin-neuraminidase (HN) and precursor fusion proteins (F0) showed them to have only a single amino acid difference: those with an ICPI value < 0.7 had proline at amino acid position 453 of the F0 protein, and those with an ICPI value > 0.7 contained a serine. The virus with an ICPI of 0.025 was further passaged, and the ICPI of non-cloned virus increased to 0.76/0.79, which was then reduced to 0.49 on cloning. The proline at residue 453 was retained, but there were two nucleotide changes in the virus of ICPI 0.49, T -> C at position 1769 in the untranslated region of the fusion gene and G -> A at position 437 of the HN gene, resulting in the amino acid change G -> R at position 116 in the HN protein.
|Item Type:||Journal Article|
|Subjects:||Q Science > QR Microbiology > QR355 Virology|
|Divisions:||Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)|
|Journal or Publication Title:||Archives of Virology|
|Number of Pages:||8|
|Page Range:||pp. 1575-1582|
|Access rights to Published version:||Restricted or Subscription Access|
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