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Ruthenium purple-mediated microelectrode biosensors based on sol-gel film
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Tian, Faming, Llaudet, Enrique and Dale, Nicholas (2007) Ruthenium purple-mediated microelectrode biosensors based on sol-gel film. Analytical Chemistry, Vol.79 (No.17). pp. 6760-6766. doi:10.1021/ac070822f
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Official URL: http://dx.doi.org/10.1021/ac070822f
Abstract
Ruthenium Purple (RP), an analogue of Prussian Blue, has potentially advantageous electrochemical characteristics. We now demonstrate its use in microelectrode biosensors for the first time. An RP layer was grown on, and remained stably anchored to, the surface of gold microelectrodes at physiological pH ranges. Crucially, it retained its electrochemical activity in sodium-based phosphate buffers. The RP microelectrodes displayed electrocatalytic reduction of hydrogen peroxide at 0 to -50 mV (vs Ag/AgCl). To fabricate biosensors on the RP microelectrodes, we used a sol-gel film electrodeposition technique to create ATP and hypoxanthine biosensors as examples of the methodology. These RP-mediated biosensors displayed excellent performance including the following: high selectivity against interferences such as 5HT, ascorbic acid, urate, and acetaminophen; high sensitivity with wide linear calibration range; and good stability. These attractive characteristics demonstrate that RP can be universally employed as an electron mediator in fabrication of highly selective oxidase-based microelectrode biosensors. Furthermore, given their ability to operate in the presence of physiological levels of Na+, the RP-mediated biosensors can be potentially applied to the in vitro and in vivo measurement of physiological signaling substances.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QD Chemistry | ||||
| Divisions: | Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010) | ||||
| Journal or Publication Title: | Analytical Chemistry | ||||
| Publisher: | American Chemical Society | ||||
| ISSN: | 0003-2700 | ||||
| Official Date: | 1 September 2007 | ||||
| Dates: |
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| Volume: | Vol.79 | ||||
| Number: | No.17 | ||||
| Number of Pages: | 7 | ||||
| Page Range: | pp. 6760-6766 | ||||
| DOI: | 10.1021/ac070822f | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
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