Holland, Richard J. (2009) The use of recently developed mass spectrometry approaches for the characterisation of biological mixtures. PhD thesis, University of Warwick.

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Abstract

The thesis describes a number of examples of the use of recently developed mass
spectrometry experimental approaches to characterise biologically important
mixtures. The recently introduced field of ambient ionisation mass spectrometry has
been utilised in the rapid, sensitive, information rich characterisation of
pharmaceutical formulations. Little, or no, sample treatment was required and the
experiments were shown to provide detailed information on active ingredients in the
presence of a number of other components. A number of ambient ionisation
approaches including DART, DESI and DAPCI were compared and advantages and
disadvantages of each approach outlined and discussed.
The exciting technology of ion mobility has recently been commercially interfaced
with mass spectrometry (IMMS). This has been utilised in a series of fundamental
experiments that probe the interaction of varied cations with isomeric oligomers of
carbohydrates. The approach enables conformational changes to be rapidly measured
over a wide (500-6000 Da) mass range. Changes in conformations were observed for
multiply cationised species which agree with previously measured solution phase
measurements.
The IMMS approach has also been used successfully to characterise a number of Nlinked
glycans released from glycoproteins. The experiments enable isomeric
structures to be differentiated and present an opportunity to develop a rapid, high
information content screen. Estimated cross sectional measurements have been
calculated and found to be in good agreement with those obtained from conventional
drift cell approaches.

Item Type:	Thesis or Dissertation (PhD)
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Library of Congress Subject Headings (LCSH):	Mass spectrometry -- Research, Drugs -- Research, Ion mobility spectroscopy
Official Date:	September 2009
Dates:	Date Event September 2009 Submitted
Institution:	University of Warwick
Theses Department:	Department of Biological Sciences
Thesis Type:	PhD
Publication Status:	Unpublished
Supervisor(s)/Advisor:	Scrivens, James H.
Format of File:	pdf
Extent:	178 leaves : ill., charts
Language:	eng
URI:	http://wrap.warwick.ac.uk/id/eprint/3154

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