Neufeld, Josh D., Wagner, Michael and Murrell, J. C. (J. Colin) (2007) Who eats what, where and when? Isotope-labelling experiments are coming of age. ISME Journal, Vol.1 (No.2). pp. 103-110. ISSN 1751-7362

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Abstract

Isotope-labelling experiments have changed the way microbial ecologists investigate the ecophysiology of microbial populations and cells in the environment. Insight into the 'uncultivated majority' accompanies methodology that involves the incorporation of stable isotopes or radioisotopes into sub-populations of environmental samples. Subsequent analysis of labelled biomarkers of sub-populations with stable-isotope probing (DNA-SIP, RNA-SIP, phospholipid-derived fatty acid-SIP) or individual cells with a combination of fluorescence in situ hybridization and microautoradiography reveals linked phylogenetic and functional information about the organisms that assimilated these compounds. Here, we review some of the most recent literature, with an emphasis on methodological improvements to the sensitivity and utility of these methods. We also highlight related isotope techniques that are in continued development and hold promise to transform the way we link phylogeny and function in complex microbial communities.

Item Type:	Journal Item
Subjects:	Q Science > QH Natural history > QH301 Biology Q Science > QR Microbiology
Divisions:	Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Journal or Publication Title:	ISME Journal
Publisher:	Nature Publishing Group
ISSN:	1751-7362
Date:	June 2007
Volume:	Vol.1
Number:	No.2
Number of Pages:	8
Page Range:	pp. 103-110
Identification Number:	10.1038/ismej.2007.30
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
URI:	http://wrap.warwick.ac.uk/id/eprint/31565

Data sourced from Thomson Reuters' Web of Knowledge

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