Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

Function and stability of abscisic acid acyl hydrazone conjugates by LC-MS2 of ex vivo samples

Tools
- Tools
+ Tools

Smith, Timothy R., Clark, Andrew J., Napier, R. (Richard), Taylor, Paul C., Thompson, Andrew J. and Marsh, Andrew (2007) Function and stability of abscisic acid acyl hydrazone conjugates by LC-MS2 of ex vivo samples. Bioconjugate Chemistry, Vol.18 (No.4). pp. 1355-1359. doi:10.1021/bc070069x

Research output not available from this repository, contact author.
Official URL: http://dx.doi.org/10.1021/bc070069x

Request Changes to record.

Abstract

We prepare a biotinylated conjugate of the ubiquitous plant hormone (S)-(+)-abscisic acid via an acyl hydrazone linkage at the C4' position and demonstrate in vivo cleavage of the otherwise stable acyl hydrazone linkage using LC-MS2. As part of a wider chemical genomic study, biological activity of the conjugate was assessed using standard epidermal peel and gravimetric transpiration assays, showing significant activity but at a level lower than the unconjugated hormone. When deuterated samples of the conjugate were fed to the plant, however, it was apparent by LC-MS2 experiments that significant levels of hydrolysis of the acyl hydrazone had taken place, contrary to in vitro stability assays in artificial sap. We conclude that abscisic acid is liberated in sufficient quantities to account for the observed physiological response and that LC-MS2 monitoring of conjugates is a simple and practical method by which such events may be assessed, whether in plants or other organisms.

Item Type: Journal Article
Subjects: Q Science > QD Chemistry
Divisions: Faculty of Science > Chemistry
Faculty of Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)
Journal or Publication Title: Bioconjugate Chemistry
Publisher: American Chemical Society
ISSN: 1043-1802
Official Date: July 2007
Dates:
DateEvent
July 2007Published
Volume: Vol.18
Number: No.4
Number of Pages: 5
Page Range: pp. 1355-1359
DOI: 10.1021/bc070069x
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access

Data sourced from Thomson Reuters' Web of Knowledge

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item
twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us