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Raised serum, adipocyte, and adipose tissue retinol-binding protein 4 in overweight women with polycystic ovary syndrome: effects of gonadal and adrenal steroids
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Tan, Bee K., Chen, Jing, Lehnert, Hendrik, Kennedy, Richard and Randeva, Harpal S. (2007) Raised serum, adipocyte, and adipose tissue retinol-binding protein 4 in overweight women with polycystic ovary syndrome: effects of gonadal and adrenal steroids. Journal of Clinical Endocrinology & Metabolism , Vol.92 (No.7). pp. 2764-2772. doi:10.1210/jc.2007-0091
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Official URL: http://dx.doi.org/10.1210/jc.2007-0091
Abstract
Context: Polycystic ovary syndrome (PCOS) is associated with insulin resistance and obesity. Recent studies have shown that serum retinol-binding protein 4 (RBP4) levels increase with obesity. Currently, no data exist on the relative expression of RBP4 in either serum or adipose tissue of PCOS women.
Objectives: mRNA expression of RBP4 from sc and omental (om) adipose tissue and sc adipocytes in overweight PCOS women were compared with matched controls; RBP4 protein in adipose tissue and serum RBP4 levels were also assessed. Additionally, we studied the effects of testosterone, 17 beta-estradiol, androstenedione, and dehydroepiandrosterone sulfate on RBP4 expression in adipose tissue explants. Design: Real-time RT-PCR and Western blotting were used to assess the relative mRNA and protein expression of RBP4. Biochemical measurements were also performed.
Results: Compared with controls, there was significant up- regulation of RBP4 mRNA in sc (P < 0.05) and om (P < 0.01) adipose tissue as well as isolated sc adipocytes (P < 0.01) of PCOS women. In addition to elevated serum RBP4 levels in PCOS women (P < 0.05), RBP4 protein levels were significantly greater in sc and om adipose tissue of PCOS women (P < 0.05 and P < 0.05, respectively). Furthermore, in human sc and om adipose tissue explants, 17 beta-estradiol significantly increased RBP4 mRNA expression, protein levels, and secretion into the culture media (P < 0.05).
Conclusions: The precise reason for elevated levels of RBP4 in overweight PCOS women is unknown, but it appears that 17 beta-estradiol may play a role in their regulation in adipose tissue.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | R Medicine > RC Internal medicine | ||||
| Divisions: | Faculty of Medicine > Warwick Medical School | ||||
| Journal or Publication Title: | Journal of Clinical Endocrinology & Metabolism | ||||
| Publisher: | Endocrine Society | ||||
| ISSN: | 0021-972x | ||||
| Official Date: | July 2007 | ||||
| Dates: |
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| Volume: | Vol.92 | ||||
| Number: | No.7 | ||||
| Number of Pages: | 9 | ||||
| Page Range: | pp. 2764-2772 | ||||
| DOI: | 10.1210/jc.2007-0091 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
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