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Homologous cpn60 genes in Rhizobium leguminosarum are not functionally equivalent
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Gould, Phillip S., Burglar, Helen R. and Lund, Peter A. (2007) Homologous cpn60 genes in Rhizobium leguminosarum are not functionally equivalent. Cell Stress & Chaperones, Volume 12 (Number 2). pp. 123-131. doi:10.1379/CSC-227R.1 ISSN 1355-8145.
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Abstract
Many bacteria possess 2 or more genes for the chaperonin GroEL and the cochaperonin GroES. In particular, rhizobial species often have multiple groEL and groES genes, with a high degree of amino-acid similarity, in their genomes. The Rhizobium leguminosarum strain A34 has 3 complete groE operons, which we have named cpn.1, cpn.2 and cpn.3. Previously we have shown the cpn.1 operon to be essential for growth, but the two other cpn operons to be dispensable. Here, we have investigated the extent to which loss of the essential GroEL homologue Cpn60.1 can be compensated for by expression of the other two GroEL homologues (Cnp60.2 and Cpn60.3). Cpn60.2 could not be overexpressed to high levels in R. leguminosarum, and was unable to replace Cpn60.1. A strain that overexpressed Cpn60.3 grew in the absence of Cpn60.1, but the complemented strain displayed a temperature-sensitive phenotype. Cpn60.1 and Cpn60.3, when coexpressed in Escherichia coli, preferentially selfassembled rather than forming mixed heteroligomers. We conclude that, despite their high amino acid similarity, the GroEL homologues of R. leguminosarum are not functionally equivalent in vivo.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QH Natural history > QH301 Biology | ||||
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School | ||||
| Journal or Publication Title: | Cell Stress & Chaperones | ||||
| Publisher: | Springer Netherlands | ||||
| ISSN: | 1355-8145 | ||||
| Official Date: | 2007 | ||||
| Dates: |
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| Volume: | Volume 12 | ||||
| Number: | Number 2 | ||||
| Number of Pages: | 9 | ||||
| Page Range: | pp. 123-131 | ||||
| DOI: | 10.1379/CSC-227R.1 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
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