HA-1 mismatch has significant effect in chronic allograft nephropathy in clinical renal transplantation
Krishnan, N. S., Higgins, R. M., Lam, F. T., Kashi, H., Jobson, S., Ramaiyan, K., Rahman, M. and Morris, A. G. (Alan George). (2007) HA-1 mismatch has significant effect in chronic allograft nephropathy in clinical renal transplantation. Transplantation Proceedings, Vol.39 (No.5). pp. 1439-1445. ISSN 0041-1345Full text not available from this repository.
Official URL: http://dx.doi.org/10.1016/j.transproceed.2007.02.0...
Background. The minor histocompatibility antigen HA-1 occurs in two allelic forms: H and R. The HA-1(H) form presented in the context of HLA A2 can elicit specific cytotoxic lymphocyte (CTL) responses and can cause graft-versus-host disease in marrow transplants. However, its significance in solid organ transplants is unknown. We determined whether incompatibility of the HA-1 resulted in enhanced rejection and whether HA-1 specific CTLs were generated.
Materials and methods. HLA A2-matched donor/recipient pairs were selected and typed for HA-1 antigens by polymerase chain reaction. Nineteen of 81 pairs were mismatched for HA-1. Peripheral blood mononuclear leucocytes from five recipients, HLA A2 DR-matched with donors, were stimulated for 3 days with third-party donor, matched for HLA A2 DR but mismatched for HA-1. Cells were stained for surface markers, HA-1(H)-specific tetramer reagent, and analyzed by flow cytometry. Controls were unstimulated cells; PBML from two patients never exposed to HA-1(H); immunoglobulin G isotype-matched controls. For all patients, acute rejection rates posttransplant was ascertained. Long-term data was available for 36 patients.
Results and conclusions. There was no difference in acute rejection rates between the HA-1-matched and -mismatched groups, but there was a significant difference in chronic rejection rates, evidenced by increased graft failures during the follow-up period (P = .0024). Lymphocytes from five HA-1-mismatched recipients were stimulated in vitro with cells from HLA-A2 and DR-matched but HA-1-mismatched surrogate donor. Though there seemed to be an excess of tetramer-positive cells, anti-HA-1-specific CTL responses were not conclusively elicited in vitro.
|Item Type:||Journal Article|
|Subjects:||Q Science > QR Microbiology > QR180 Immunology
R Medicine > RD Surgery
|Divisions:||Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)|
|Journal or Publication Title:||Transplantation Proceedings|
|Official Date:||June 2007|
|Number of Pages:||7|
|Page Range:||pp. 1439-1445|
|Access rights to Published version:||Restricted or Subscription Access|
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