Specific Rab GTPase-activating proteins define the Shiga toxin and epidermal growth factor uptake pathways
Fuchs, Evelyn, Haas, Alexander K., Spooner, Robert A. , Yoshimura, Shin-Ichiro, Lord, Mike (J. Mike) and Barr, Francis A.. (2007) Specific Rab GTPase-activating proteins define the Shiga toxin and epidermal growth factor uptake pathways. Journal of Cell Biology, Vol.177 (No.6). pp. 1133-1143. ISSN 0021-9525Full text not available from this repository.
Official URL: http://dx.doi.org/10.1083/jcb.200612068
R ab family guanosine triphosphatases ( GTPases) together with their regulators define specific pathways of membrane traffic within eukaryotic cells. In this study, we have investigated which Rab GTPase-activating proteins ( GAPs) can interfere with the trafficking of Shiga toxin from the cell surface to the Golgi apparatus and studied transport of the epidermal growth factor ( EGF) from the cell surface to endosomes. This screen identifies 6 ( EVI5, RN-tre/USP6NL, TBC1D10A-C, and TBC1D17) of 39 predicted human Rab GAPs as specific regulators of Shiga toxin but not EGF uptake. We show that Rab43 is the target of RN- tre and is required for Shiga toxin uptake. In contrast, RabGAP- 5, a Rab5 GAP, was unique among the GAPs tested and reduced the uptake of EGF but not Shiga toxin. These results suggest that Shiga toxin trafficking to the Golgi is a multistep process controlled by several Rab GAPs and their target Rabs and that this process is discrete from ligand-induced EGF receptor trafficking.
|Item Type:||Journal Article|
|Subjects:||Q Science > QH Natural history > QH301 Biology|
|Divisions:||Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)|
|Journal or Publication Title:||Journal of Cell Biology|
|Publisher:||Rockefeller University Press|
|Official Date:||18 June 2007|
|Number of Pages:||11|
|Page Range:||pp. 1133-1143|
|Access rights to Published version:||Restricted or Subscription Access|
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