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Specific Rab GTPase-activating proteins define the Shiga toxin and epidermal growth factor uptake pathways

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Fuchs, Evelyn, Haas, Alexander K., Spooner, Robert A. , Yoshimura, Shin-Ichiro, Lord, Mike and Barr, Francis A. (2007) Specific Rab GTPase-activating proteins define the Shiga toxin and epidermal growth factor uptake pathways. Journal of Cell Biology, Vol.177 (No.6). pp. 1133-1143. doi:10.1083/jcb.200612068

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Official URL: http://dx.doi.org/10.1083/jcb.200612068

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Abstract

R ab family guanosine triphosphatases ( GTPases) together with their regulators define specific pathways of membrane traffic within eukaryotic cells. In this study, we have investigated which Rab GTPase-activating proteins ( GAPs) can interfere with the trafficking of Shiga toxin from the cell surface to the Golgi apparatus and studied transport of the epidermal growth factor ( EGF) from the cell surface to endosomes. This screen identifies 6 ( EVI5, RN-tre/USP6NL, TBC1D10A-C, and TBC1D17) of 39 predicted human Rab GAPs as specific regulators of Shiga toxin but not EGF uptake. We show that Rab43 is the target of RN- tre and is required for Shiga toxin uptake. In contrast, RabGAP- 5, a Rab5 GAP, was unique among the GAPs tested and reduced the uptake of EGF but not Shiga toxin. These results suggest that Shiga toxin trafficking to the Golgi is a multistep process controlled by several Rab GAPs and their target Rabs and that this process is discrete from ligand-induced EGF receptor trafficking.

Item Type: Journal Article
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Journal or Publication Title: Journal of Cell Biology
Publisher: Rockefeller University Press
ISSN: 0021-9525
Official Date: 18 June 2007
Dates:
DateEvent
18 June 2007Published
Volume: Vol.177
Number: No.6
Number of Pages: 11
Page Range: pp. 1133-1143
DOI: 10.1083/jcb.200612068
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access

Data sourced from Thomson Reuters' Web of Knowledge

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