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Specific Rab GTPase-activating proteins define the Shiga toxin and epidermal growth factor uptake pathways
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Fuchs, Evelyn, Haas, Alexander K., Spooner, Robert A. , Yoshimura, Shin-Ichiro, Lord, Mike (J. Mike) and Barr, Francis A.. (2007) Specific Rab GTPase-activating proteins define the Shiga toxin and epidermal growth factor uptake pathways. Journal of Cell Biology, Vol.177 (No.6). pp. 1133-1143. ISSN 0021-9525
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Official URL: http://dx.doi.org/10.1083/jcb.200612068
Abstract
R ab family guanosine triphosphatases ( GTPases) together with their regulators define specific pathways of membrane traffic within eukaryotic cells. In this study, we have investigated which Rab GTPase-activating proteins ( GAPs) can interfere with the trafficking of Shiga toxin from the cell surface to the Golgi apparatus and studied transport of the epidermal growth factor ( EGF) from the cell surface to endosomes. This screen identifies 6 ( EVI5, RN-tre/USP6NL, TBC1D10A-C, and TBC1D17) of 39 predicted human Rab GAPs as specific regulators of Shiga toxin but not EGF uptake. We show that Rab43 is the target of RN- tre and is required for Shiga toxin uptake. In contrast, RabGAP- 5, a Rab5 GAP, was unique among the GAPs tested and reduced the uptake of EGF but not Shiga toxin. These results suggest that Shiga toxin trafficking to the Golgi is a multistep process controlled by several Rab GAPs and their target Rabs and that this process is discrete from ligand-induced EGF receptor trafficking.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010) |
| Journal or Publication Title: | Journal of Cell Biology |
| Publisher: | Rockefeller University Press |
| ISSN: | 0021-9525 |
| Official Date: | 18 June 2007 |
| Volume: | Vol.177 |
| Number: | No.6 |
| Number of Pages: | 11 |
| Page Range: | pp. 1133-1143 |
| Identification Number: | 10.1083/jcb.200612068 |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Restricted or Subscription Access |
| URI: | http://wrap.warwick.ac.uk/id/eprint/31789 |
Data sourced from Thomson Reuters' Web of Knowledge
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