Avian metapneumovirus SH gene end and G protein mutations influence the level of protection of live-vaccine candidates
Naylor, Clive J., Ling, Roger, Edworthy, Nicole Lynn, Savage, Carol E. and Easton, A. J. (Andrew J.). (2007) Avian metapneumovirus SH gene end and G protein mutations influence the level of protection of live-vaccine candidates. Journal of General Virology, Vol.88 (No.6). pp. 1767-1775. ISSN 0022-1317Full text not available from this repository.
Official URL: http://dx.doi.org/10.1099/vir.0.82755-0
A prototype avian metapneumovirus (AMPV) vaccine (P20) was previously shown to give variable outcomes in experimental trials. Following plaque purification, three of 12 viruses obtained from P20 failed to induce protection against virulent challenge, whilst the remainder retained their protective capacity. The genome sequences of two protective viruses were identical to the P20 consensus, whereas two non-protective viruses differed only in the SH gene transcription termination signal. Northern blotting showed that the alterations in the SH gene-end region of the non-protective viruses led to enhanced levels of dicistronic mRNA produced by transcriptional readthrough. A synthetic minigenome was used to demonstrate that the altered SH gene-end region reduced the level of protein expression from a downstream gene. The genomes of the remaining eight plaque-purified viruses were sequenced in the region where the P20 consensus sequence differed from the virulent progenitor. The seven protective clones were identical, whereas the non-protective virus retained the virulent progenitor sequence at two positions and contained extensive alterations in its attachment (G) protein sequence associated with a reduced or altered expression pattern of G protein on Western blots. The data indicate that the efficacy of a putative protective vaccine strain is affected by mutations altering the balance of G protein expression.
|Item Type:||Journal Article|
|Subjects:||T Technology > TP Chemical technology
Q Science > QR Microbiology > QR355 Virology
|Divisions:||Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)|
|Journal or Publication Title:||Journal of General Virology|
|Publisher:||Society for General Microbiology|
|Number of Pages:||9|
|Page Range:||pp. 1767-1775|
|Access rights to Published version:||Restricted or Subscription Access|
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