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Methodological considerations for the use of stable isotope probing in microbial ecology
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Neufeld, Josh D., Dumont, Marc G., Vohra, Jyotsna and Murrell, J. C. (J. Colin) (2007) Methodological considerations for the use of stable isotope probing in microbial ecology. Microbial Ecology, Vol.53 (No.3). pp. 435-442. doi:10.1007/s00248-006-9125-x ISSN 0095-3628.
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Official URL: http://dx.doi.org/10.1007/s00248-006-9125-x
Abstract
Stable isotope probing (SIP) is a method used for labeling uncultivated microorganisms in environmental samples or directly in field studies using substrate enriched with stable isotope (e.g., C-13). After consumption of the substrate, the cells of microorganisms that consumed the substrate become enriched in the isotope. Labeled biomarkers, such as phospholipid-derived fatty acid (PLFA), ribosomal RNA, and DNA can be analyzed with a range of molecular and analytical techniques, and used to identify and characterize the organisms that incorporated the substrate. The advantages and disadvantages of PLFA-SIP, RNA-SIP, and DNA-SIP are presented. Using examples from our laboratory and from the literature, we discuss important methodological considerations for a successful SIP experiment.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QR Microbiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Biological Sciences ( -2010) | ||||
Journal or Publication Title: | Microbial Ecology | ||||
Publisher: | Springer | ||||
ISSN: | 0095-3628 | ||||
Official Date: | April 2007 | ||||
Dates: |
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Volume: | Vol.53 | ||||
Number: | No.3 | ||||
Number of Pages: | 8 | ||||
Page Range: | pp. 435-442 | ||||
DOI: | 10.1007/s00248-006-9125-x | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Title of Event: | Workshop on Microbial Environmental Genomics | ||||
Type of Event: | Workshop | ||||
Location of Event: | Shanghai Jiao Tong, Univ, Manhang Campus, Shanghai, People's Republic of China | ||||
Date(s) of Event: | June 12-15, 2005 |
Data sourced from Thomson Reuters' Web of Knowledge
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