Neufeld, Josh D., Dumont, Marc G., Vohra, Jyotsna and Murrell, J. C. (J. Colin). (2007) Methodological considerations for the use of stable isotope probing in microbial ecology. Microbial Ecology, Vol.53 (No.3). pp. 435-442. ISSN 0095-3628

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Abstract

Stable isotope probing (SIP) is a method used for labeling uncultivated microorganisms in environmental samples or directly in field studies using substrate enriched with stable isotope (e.g., C-13). After consumption of the substrate, the cells of microorganisms that consumed the substrate become enriched in the isotope. Labeled biomarkers, such as phospholipid-derived fatty acid (PLFA), ribosomal RNA, and DNA can be analyzed with a range of molecular and analytical techniques, and used to identify and characterize the organisms that incorporated the substrate. The advantages and disadvantages of PLFA-SIP, RNA-SIP, and DNA-SIP are presented. Using examples from our laboratory and from the literature, we discuss important methodological considerations for a successful SIP experiment.

Item Type:	Journal Article
Subjects:	Q Science > QH Natural history > QH301 Biology Q Science > QR Microbiology
Divisions:	Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Journal or Publication Title:	Microbial Ecology
Publisher:	Springer
ISSN:	0095-3628
Date:	April 2007
Volume:	Vol.53
Number:	No.3
Number of Pages:	8
Page Range:	pp. 435-442
Identification Number:	10.1007/s00248-006-9125-x
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Title of Event:	Workshop on Microbial Environmental Genomics
Type of Event:	Workshop
Location of Event:	Shanghai Jiao Tong, Univ, Manhang Campus, Shanghai, People's Republic of China
Date(s) of Event:	June 12-15, 2005
URI:	http://wrap.warwick.ac.uk/id/eprint/31980

Data sourced from Thomson Reuters' Web of Knowledge

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