Waine, David J., Henry, Deborah A., Baldwin, Adam, Speert, David P., Honeybourne, David, Mahenthiralingam, Eshwar and Dowson, Christopher G.. (2007) Reliability of multilocus sequence typing of the Burkholderia cepacia complex in cystic fibrosis. Journal of Cystic Fibrosis, Vol.6 (No.3). pp. 215-219. ISSN 1569-1993

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Abstract

Introduction: Infection with the Burkholderia cepacia complex is an important cause of morbidity and mortality in cystic fibrosis (CF). We investigated the molecular clock speed of the seven genes used in the multilocus sequence typing (MLST) scheme for these bacteria. Methods: At least two isolates, separated by months to years, from each of 20 patients were typed using MLST. In total 41 isolates, providing 128 isolate-years, were analyzed. Mutation and recombination rates were estimated assuming a Poisson distribution. Results: Out of 20 patients, 15 had no change in sequence type over time (mean 7.07 years, range 1.09 to 14.24). One patient had strain replacement. Three patients had evidence of recombination involving one of the seven housekeeping genes, and one patient had evidence of recombination of two genes. The mutation rate was estimated as 2.36 x 10(-6) per nucleotide per year (50% confidence limit) and 1.02 x 10(-5) per nucleotide per year (upper 95% confidence limit). The rate of nucleotide changes due to recombination events was estimated as 0.676 to 0.839 per year (95% confidence limits). Conclusions: B. cepacia complex housekeeping genes have a slow molecular clock speed and MLST provides a robust and reliable typing technique for isolates from this complex. A low rate of point mutation was found, with a higher rate of recombination events, in keeping with previous cross-sectional epidemiological data. The study also demonstrated, for the first time, recombination in a longitudinal in vivo study. (C) 2006 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Subjects:	R Medicine > RC Internal medicine
Divisions:	Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Journal or Publication Title:	Journal of Cystic Fibrosis
Publisher:	Elsevier Science BV
ISSN:	1569-1993
Date:	May 2007
Volume:	Vol.6
Number:	No.3
Number of Pages:	5
Page Range:	pp. 215-219
Identification Number:	10.1016/j.jcf.2006.10.003
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
URI:	http://wrap.warwick.ac.uk/id/eprint/32028

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