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Circular and linear dichroism of proteins
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Bulheller, Benjamin M., Rodger, Alison and Hirst, Jonathan D. (2007) Circular and linear dichroism of proteins. Physical Chemistry Chemical Physics, Volume 9 (Number 17). pp. 2020-2035. doi:10.1039/b615870f
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Official URL: http://dx.doi.org/10.1039/b615870f
Abstract
Circular dichroism (CD) is an important technique in the structural characterisation of proteins, and especially for secondary structure determination. The CD of proteins can be calculated from first principles using the so-called matrix method, with an accuracy which is almost quantitative for helical proteins. Thus, for proteins of unknown structure, CD calculations and experimental data can be used in conjunction to aid structure analysis. Linear dichroism (LD) can be calculated using analogous methodology and has been used to establish the relative orientations of subunits in proteins and protein orientation in an environment such as a membrane. However, simple analysis of LD data is not possible, due to overlapping transitions. So coupling the calculations and experiment is an important strategy. In this paper, the use of LD for the determination of protein orientation and how these data can be interpreted with the aid of calculations, are discussed. We review methods for the calculation of CD spectra, focusing on semiempirical and ab initio parameter sets used in the matrix method. Lastly, a new web interface for online CD and LD calculation is presented.
Item Type: | Journal Item | ||||
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Subjects: | Q Science > QD Chemistry Q Science > QC Physics |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||
Journal or Publication Title: | Physical Chemistry Chemical Physics | ||||
Publisher: | Royal Society of Chemistry | ||||
ISSN: | 1463-9076 | ||||
Official Date: | 2007 | ||||
Dates: |
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Volume: | Volume 9 | ||||
Number: | Number 17 | ||||
Number of Pages: | 16 | ||||
Page Range: | pp. 2020-2035 | ||||
DOI: | 10.1039/b615870f | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
Data sourced from Thomson Reuters' Web of Knowledge
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