Discrimination of Pseudomonas syringae isolates from sweet and wild cherry using rep-PCR
Vicente, Joana G. and Roberts, Steven J.. (2007) Discrimination of Pseudomonas syringae isolates from sweet and wild cherry using rep-PCR. European Journal of Plant Pathology, Vol.117 (No.4). pp. 383-392. ISSN 0929-1873Full text not available from this repository.
Official URL: http://dx.doi.org/10.1007/s10658-007-9107-y
Bacterial canker is one of the most important. diseases of cherry (Prunes avium). This disease can be caused. by two pathovars of Pseudomonas syringae: pv. morsprunorum and pv. syringae. Repetitive DNA polymerase chain reaction-based fingerprinting (rep-PCR) was investigated as. a. method to distinguish, pathovars, races: and isolates. of P. syringae from sweet and wild cherry. After amplification of total genomic DNA from 87 isolates using the REP (repetitive extragenic palindromic), ERIC (enterobacterial repetitive intergenic consensus) and BOX primers, followed by agarose gel electrophoresis, groups, of isolates showed specific patterns of PCR products. Pseudomonas syringae pv. syringae isolates were highly variable. The differences amongst the fingerprints of P. syringae pv. morsprunorum race 1 isolates were small. The patterns of P: syringae pv. morsprunorum race 2 isolates were also very uniform; with one exception; and distinct from: the race 1 isolates. rep-PCR is a rapid and simple method to. identify isolates. of the two races of P. syringae pv. morsprunorum; this method can also assist in the identification of P. syringae pv. syringae isolates, although it cannot replace inoculation on susceptible hosts such as cherry and lilac.
|Item Type:||Journal Article|
S Agriculture > SB Plant culture
|Divisions:||Faculty of Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)|
|Journal or Publication Title:||European Journal of Plant Pathology|
|Number of Pages:||10|
|Page Range:||pp. 383-392|
|Access rights to Published version:||Restricted or Subscription Access|
Actions (login required)