Mottershead, Marcus, Karteris, Emmanuel, Barclay, Jill Y., Suortamo, Saira, Newbold, Mark, Randeva, Harpal S. and Nwokolo, Chuka U.. (2007) Immunohistochemical and quantitative mRNA assessment of ghrelin expression in gastric and oesophageal adenocarcinoma. Journal of Clinical Pathology, Vol.60 (No.4). pp. 405-409. ISSN 0021-9746

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Abstract

Background: Ghrelin is an orexigenic gut peptide produced predominantly by the stomach. Gastric mucosal ghrelin production could be compromised by an infiltrating adenocarcinoma.

Aims: To assess the expression of ghrelin mRNA and peptide in oesophagogastric adenocarcinomas and adjacent non- neoplastic mucosa.

Methods: 10 gastric and 22 oesophageal adenocarcinoma archival samples were randomly selected from a database. The presence of ghrelin- positive cells was assessed in cancer and corresponding non- neoplastic mucosa by immunohistochemistry. Quantitative reverse transcriptase polymerase chain reaction ( PCR) for ghrelin mRNA was also performed on 24 gastric and 8 oesophageal adenocarcinoma specimens and adjacent non- neoplastic mucosa.

Results: Immunohistochemistry and reverse transcriptase PCR confirm a negligible expression of ghrelin in adenocarcinoma specimens. By contrast, non- neoplastic gastric mucosa was rich in ghrelin- positive cells and ghrelin mRNA. The number ( median and range) of ghrelin- positive cells per 2 mm section of non- neoplastic mucosa was 73 ( 45 - 215) in the corpus; this was significantly higher than in cardia mucosa ( 9 ( 0 - 64), p, 0.001) and antral mucosa ( 5 ( 0 - 14), p, 0.001).

Conclusions: Gastric and oesophageal adenocarcinomas have no ghrelin- producing cells. The highest level of ghrelin expression was noted in the non- neoplastic mucosa of the gastric corpus. Disruption of the gastric ghrelin- producing mechanism may occur during oesophagogastric malignancy.

Item Type:	Journal Article
Subjects:	R Medicine > RB Pathology
Divisions:	Faculty of Medicine > Warwick Medical School
Journal or Publication Title:	Journal of Clinical Pathology
Publisher:	B M J Group
ISSN:	0021-9746
Official Date:	April 2007
Volume:	Vol.60
Number:	No.4
Number of Pages:	5
Page Range:	pp. 405-409
Identification Number:	10.1136/jcp.2006.038356
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
URI:	http://wrap.warwick.ac.uk/id/eprint/32129

Data sourced from Thomson Reuters' Web of Knowledge

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