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Immunohistochemical and quantitative mRNA assessment of ghrelin expression in gastric and oesophageal adenocarcinoma
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Mottershead, Marcus, Karteris, Emmanuel, Barclay, Jill Y., Suortamo, Saira, Newbold, Mark, Randeva, Harpal S. and Nwokolo, Chuka U. (2007) Immunohistochemical and quantitative mRNA assessment of ghrelin expression in gastric and oesophageal adenocarcinoma. Journal of Clinical Pathology, Vol.60 (No.4). pp. 405-409. doi:10.1136/jcp.2006.038356 ISSN 0021-9746.
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Official URL: http://dx.doi.org/10.1136/jcp.2006.038356
Abstract
Background: Ghrelin is an orexigenic gut peptide produced predominantly by the stomach. Gastric mucosal ghrelin production could be compromised by an infiltrating adenocarcinoma.
Aims: To assess the expression of ghrelin mRNA and peptide in oesophagogastric adenocarcinomas and adjacent non- neoplastic mucosa.
Methods: 10 gastric and 22 oesophageal adenocarcinoma archival samples were randomly selected from a database. The presence of ghrelin- positive cells was assessed in cancer and corresponding non- neoplastic mucosa by immunohistochemistry. Quantitative reverse transcriptase polymerase chain reaction ( PCR) for ghrelin mRNA was also performed on 24 gastric and 8 oesophageal adenocarcinoma specimens and adjacent non- neoplastic mucosa.
Results: Immunohistochemistry and reverse transcriptase PCR confirm a negligible expression of ghrelin in adenocarcinoma specimens. By contrast, non- neoplastic gastric mucosa was rich in ghrelin- positive cells and ghrelin mRNA. The number ( median and range) of ghrelin- positive cells per 2 mm section of non- neoplastic mucosa was 73 ( 45 - 215) in the corpus; this was significantly higher than in cardia mucosa ( 9 ( 0 - 64), p, 0.001) and antral mucosa ( 5 ( 0 - 14), p, 0.001).
Conclusions: Gastric and oesophageal adenocarcinomas have no ghrelin- producing cells. The highest level of ghrelin expression was noted in the non- neoplastic mucosa of the gastric corpus. Disruption of the gastric ghrelin- producing mechanism may occur during oesophagogastric malignancy.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | R Medicine > RB Pathology | ||||
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School | ||||
| Journal or Publication Title: | Journal of Clinical Pathology | ||||
| Publisher: | B M J Group | ||||
| ISSN: | 0021-9746 | ||||
| Official Date: | April 2007 | ||||
| Dates: |
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| Volume: | Vol.60 | ||||
| Number: | No.4 | ||||
| Number of Pages: | 5 | ||||
| Page Range: | pp. 405-409 | ||||
| DOI: | 10.1136/jcp.2006.038356 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
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