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Dynamic interplay between the neutral glycosphingolipid CD77/Gb3 and the therapeutic antibody target CD20 within the lipid bilayer of model B lymphoma cells
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Jarvis, Rosemary M., Chamba, Anita, Holder, Michelle J., Challa, Anita, Smith, Daniel C., Hodgkin, Matthew N., Lord, Mike (J. Mike) and Gordon, John. (2007) Dynamic interplay between the neutral glycosphingolipid CD77/Gb3 and the therapeutic antibody target CD20 within the lipid bilayer of model B lymphoma cells. Biochemical and Biophysical Research Communications, Vol.355 (No.4). pp. 944-949. ISSN 0006-291X
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Official URL: http://dx.doi.org/10.1016/j.bbrc.2007.02.053
Abstract
The centroblast-specific differentiation marker CD77 (Gb(3)), is the receptor for Shiga-like toxin (SLT). The dynamic relationship between Gb(3)/CD77 and key B-cell membrane proteins was studied in Burkitt's lymphoma cells with a focus on CD20. Engagement of Gb3/CD77 with SLT-B reduced the amount of CD20 and CXCR4 available, but levels of BCR, MHC Class 11, CD21, CD27 and CD54 remained unchanged. Cholesterol depletion promoted a decrease in the number of sites accessed by CD20, CXCR4 and Gb(3)/ CD77 antibodies. Constitutive localisation of Gb3/CD77 to lipid rafts was unperturbed by either SLT-B binding or cholesterol depletion, whereas the opposite was true for CD20. The effects were specific to SLT-B, highlighted by the inability of cholera toxin B-subunit to alter CD20 availability. Thus, the binding of Gb3/CD77 by its cognate ligand transmits information within the lipid bilayer of model lymphoma cells to impact the behaviour of selective proteins, most notably CD20, via a mechanism influenced by the level of cholesterol within the membrane. (c) 2007 Elsevier Inc. All rights reserved.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010) |
| Journal or Publication Title: | Biochemical and Biophysical Research Communications |
| Publisher: | Elsevier |
| ISSN: | 0006-291X |
| Date: | 20 April 2007 |
| Volume: | Vol.355 |
| Number: | No.4 |
| Number of Pages: | 6 |
| Page Range: | pp. 944-949 |
| Identification Number: | 10.1016/j.bbrc.2007.02.053 |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Restricted or Subscription Access |
| URI: | http://wrap.warwick.ac.uk/id/eprint/32213 |
Data sourced from Thomson Reuters' Web of Knowledge
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