Dynamic interplay between the neutral glycosphingolipid CD77/Gb3 and the therapeutic antibody target CD20 within the lipid bilayer of model B lymphoma cells
Jarvis, Rosemary M., Chamba, Anita, Holder, Michelle J., Challa, Anita, Smith, Daniel C., Hodgkin, Matthew N., Lord, Mike (J. Mike) and Gordon, John. (2007) Dynamic interplay between the neutral glycosphingolipid CD77/Gb3 and the therapeutic antibody target CD20 within the lipid bilayer of model B lymphoma cells. Biochemical and Biophysical Research Communications, Vol.355 (No.4). pp. 944-949. ISSN 0006-291XFull text not available from this repository.
Official URL: http://dx.doi.org/10.1016/j.bbrc.2007.02.053
The centroblast-specific differentiation marker CD77 (Gb(3)), is the receptor for Shiga-like toxin (SLT). The dynamic relationship between Gb(3)/CD77 and key B-cell membrane proteins was studied in Burkitt's lymphoma cells with a focus on CD20. Engagement of Gb3/CD77 with SLT-B reduced the amount of CD20 and CXCR4 available, but levels of BCR, MHC Class 11, CD21, CD27 and CD54 remained unchanged. Cholesterol depletion promoted a decrease in the number of sites accessed by CD20, CXCR4 and Gb(3)/ CD77 antibodies. Constitutive localisation of Gb3/CD77 to lipid rafts was unperturbed by either SLT-B binding or cholesterol depletion, whereas the opposite was true for CD20. The effects were specific to SLT-B, highlighted by the inability of cholera toxin B-subunit to alter CD20 availability. Thus, the binding of Gb3/CD77 by its cognate ligand transmits information within the lipid bilayer of model lymphoma cells to impact the behaviour of selective proteins, most notably CD20, via a mechanism influenced by the level of cholesterol within the membrane. (c) 2007 Elsevier Inc. All rights reserved.
|Item Type:||Journal Article|
|Subjects:||Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
|Divisions:||Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)|
|Journal or Publication Title:||Biochemical and Biophysical Research Communications|
|Date:||20 April 2007|
|Number of Pages:||6|
|Page Range:||pp. 944-949|
|Access rights to Published version:||Restricted or Subscription Access|
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