slowmo is required for Drosophila germline proliferation
Reeve, Simon, Carhan, Ahmet, Dee, Chris T. and Moffat, Kevin G.. (2007) slowmo is required for Drosophila germline proliferation. Genesis: The Journal of Genetics and Development, Vol.45 (No.2). pp. 66-75. ISSN 1526-954XFull text not available from this repository.
Official URL: http://dx.doi.org/10.1002/dvg.20265
Null mutations in the Drosophila gene, slowmo (slmo), result in reduced mobility and lethality in first-instar larvae. Slowmo encodes a mitochondrial protein of unknown function, as do the two other homologs found in Drosophila. Here, we have studied a hypomorphic Pelement allele of slmo demonstrating its effects on germline divisions in both testes and ovaries. Using in situ studies, enhancer-trap activity, and promoter fusions, we have shown that simo expression in testes is found in the somatic cyst cells (SCC). The hypomorphic allele for Slmo revealed apoptotic loss of germline cells in the larval germline, culminating in a complete absence of the germline in adult flies. In females, a similar degeneration of the germarium is observed, while reporter gene expression is found in both germline and somatic cells. Using a null mutation in female germline clones, we find slmo is dispensable from the germline cells. Our results suggest that Slowmo is not required in germline cells directly, but is required in SCCs responsible for maintaining germline survival in both sexes.
|Item Type:||Journal Article|
|Subjects:||Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
|Divisions:||Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)|
|Journal or Publication Title:||Genesis: The Journal of Genetics and Development|
|Publisher:||John Wiley & Sons Ltd.|
|Number of Pages:||10|
|Page Range:||pp. 66-75|
|Access rights to Published version:||Restricted or Subscription Access|
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