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Improved curve fitting procedures to determine equilibrium binding constants
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Stootman, Frank H., Fisher, Dianne M., Rodger, Alison and Aldrich-Wright, Janice R. (2006) Improved curve fitting procedures to determine equilibrium binding constants. ANALYST, 131 (10). pp. 1145-1151. doi:10.1039/b604686j ISSN 0003-2654.
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Official URL: http://dx.doi.org/10.1039/b604686j
Abstract
For ligand - biomacromolecule titration experiments it has been traditional practice to extract parameters such as the equilibrium binding constant K and the number of bases per ligand binding site n with relatively labour intensive methods, usually based on single wavelength data, such as the difference method by Rodger and Norden coupled together with a Scatchard plot. Presented in this paper are both the theory and a least squares fitting method to derive parameters such as K and n more directly from all spectral non-linear experimental data. Both the case of non competitive binding of a metal complex ligand to DNA and the case of displacement by a metal complex ligand of an ethidium marker attached to the DNA are considered. This work may be applied directly to reduce experimental data produced by a spectropolarimeter ( for circular or linear dichroism) or a spectrophotometer ( for fluorescence or UV-Vis spectroscopy).
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QD Chemistry | ||||
Journal or Publication Title: | ANALYST | ||||
Publisher: | ROYAL SOC CHEMISTRY | ||||
ISSN: | 0003-2654 | ||||
Official Date: | 2006 | ||||
Dates: |
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Volume: | 131 | ||||
Number: | 10 | ||||
Number of Pages: | 7 | ||||
Page Range: | pp. 1145-1151 | ||||
DOI: | 10.1039/b604686j | ||||
Publication Status: | Published |
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