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Interaction of PLD1b with actin in antigen-stimulated mast cells
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Farquhar, M. J., Powner, D. J., Levine, B. A., Wright, M. H., Ladds, G. and Hodgkin, M. N.. (2007) Interaction of PLD1b with actin in antigen-stimulated mast cells. Cellular Signalling, Vol.19 (No.2). pp. 349-358. ISSN 0898-6568
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Official URL: http://dx.doi.org/10.1016/j.cellsig.2006.07.016
Abstract
Phosphatidic acid, the product of phospholipase D catalysed phosphatidylcholine hydrolysis is an important signalling molecule that has been implicated in regulation of actin cytoskeleton remodelling and secretion from mast cells. We show that human PLD1b (hPLD1b) is an actin-binding protein and the N-terminus is predominantly involved in this interaction. Protein kinase C (PKC) is a major upstream regulator of PLD activity and PKC phosphorylation sites have been identified within the N-terminus of PLDIb at serine 2 and threonine 147. Over-expression of wild type hPLD1b in mast cells showed that antigen stimulation significantly enhanced co-localisation of PLD1b with actin structures. Mutation of serine 2 to alanine abolished antigen-induced co-localisation whereas mutation of threonine 147 had less dramatic effects on co-localisation. The absence of co-localisation of PLDIb (S2A) with actin coincides with a significant decrease in PLD activity in cells expressing the PLD1b (S2A) mutant. In resting RBL-2H3 cells, mutation of serine 2 to aspartate resulted in constitutive co-localisation of PLD with the actin cytoskeleton, coincident with restored PLD activity. These results reveal that serine 2 is an important regulatory site involved in controlling PLD enzyme activity and the interaction between PLD and actin. (c) 2006 Elsevier Inc. All rights reserved.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010) |
| Journal or Publication Title: | Cellular Signalling |
| Publisher: | Elsevier |
| ISSN: | 0898-6568 |
| Date: | February 2007 |
| Volume: | Vol.19 |
| Number: | No.2 |
| Number of Pages: | 10 |
| Page Range: | pp. 349-358 |
| Identification Number: | 10.1016/j.cellsig.2006.07.016 |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Restricted or Subscription Access |
| URI: | http://wrap.warwick.ac.uk/id/eprint/32464 |
Data sourced from Thomson Reuters' Web of Knowledge
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