Kyuno, Jun-ichi and Jones, Elizabeth A.. (2007) GDNF expression during Xenopus development. GENE EXPRESSION PATTERNS, 7 (3). pp. 313-317. ISSN 1567-133X

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Abstract

Glial cell line-derived neurotrophic factor (GDNF) has multiple roles in kidney morphogenesis, spermatogenesis, and neurogenesis during development. In this study, we report the cloning and expression pattern of Xenopus laevis GDNF. The X. laevis GDNF cDNA sequence has a complete open reading frame of 684 bases, predicting 227 amino acid residues at the protein level. Comparison of the X. laevis GDNF amino acid sequence with those of chick, human, Mouse, rat and zebrafish indicates that X. lacvis GDNF has 60%-52% and 75%-62% identity over the whole amino acid sequence and for the putative mature forms, respectively. All known functional motifs of GDNF were conserved in the X. laevis sequence. Temporal expression analysis by RT-PCR indicated that GDNF transcripts were first detectable at stage 12 at a low level, and gradually increased up to stage 22. From stage 24, the expression sharply increased and continued at a similar level as development progressed. spatial expression analysis by whole-mount in situ hybridization showed that the GDNF mRNA was predominantly detected in somites, pronephros, pharyngeal arches, epibranchial placodes, digestive tract and some of the lateral line structure. These results suggest that this X. lacvis gene is the orthologue for GDNF. (c) 2006 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Subjects:	Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH426 Genetics
Journal or Publication Title:	GENE EXPRESSION PATTERNS
Publisher:	ELSEVIER SCIENCE BV
ISSN:	1567-133X
Date:	January 2007
Volume:	7
Number:	3
Number of Pages:	5
Page Range:	pp. 313-317
Identification Number:	10.1016/j.modgep.2006.08.005
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/32505

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