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Improved growth of enteric adenovirus type 40 in a modified cell line that can no longer respond to interferon stimulation
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Sherwood, Victoria, Burgert, Hans-Gerhard , Chen, Yun-Hsiang, Sanghera, Sandeep, Katafigiotis, Socrates, Randall, Richard E., Connerton, Ian and Mellits, Kenneth H. (2007) Improved growth of enteric adenovirus type 40 in a modified cell line that can no longer respond to interferon stimulation. Journal of General Virology, Volume 88 (Number Part 1). pp. 71-76. doi:10.1099/vir.0.82445-0 ISSN 0022-1317.
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Official URL: http://dx.doi.org/10.1099/vir.0.82445-0
Abstract
Human enteric adenoviruses propagate poorly in conventional human cell lines used to grow other adenovirus serotypes. As human enteric adenoviruses have a defect in counteracting the cellular interferon (IFN) response in cell culture, to aid in growth of the virus, a 293-based cell line defective in its ability to respond to IFN was constructed. This cell line (293-SV5/V) constitutively expresses V-protein of the paramyxovirus Simian virus 5, which degrades the signal transducer and activator of transcription 1 (STAT1) and thereby prevents the STAT1-mediated IFN response. Analysis of human enteric adenovirus type 40 (HAdV-40)-infected 293-SV5/V cells compared with parental 293 cells shows that the recombinant line allows more rapid production of virus and results in higher titres. These results suggest that the defect in HAdV-40 in counteracting the IFN response can be overcome at least partially through the use of 293-SV5/V cell lines.
Item Type: | Journal Article | ||||
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Subjects: | T Technology > TP Chemical technology Q Science > QR Microbiology > QR355 Virology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Biological Sciences ( -2010) | ||||
Journal or Publication Title: | Journal of General Virology | ||||
Publisher: | Society for General Microbiology | ||||
ISSN: | 0022-1317 | ||||
Official Date: | January 2007 | ||||
Dates: |
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Volume: | Volume 88 | ||||
Number: | Number Part 1 | ||||
Number of Pages: | 6 | ||||
Page Range: | pp. 71-76 | ||||
DOI: | 10.1099/vir.0.82445-0 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
Data sourced from Thomson Reuters' Web of Knowledge
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