Improved growth of enteric adenovirus type 40 in a modified cell line that can no longer respond to interferon stimulation
Sherwood, Victoria, Burgert, Hans-Gerhard , Chen, Yun-Hsiang, Sanghera, Sandeep, Katafigiotis, Socrates, Randall, Richard E., Connerton, Ian and Mellits, Kenneth H.. (2007) Improved growth of enteric adenovirus type 40 in a modified cell line that can no longer respond to interferon stimulation. Journal of General Virology, Volume 88 (Number Part 1). pp. 71-76. ISSN 0022-1317Full text not available from this repository.
Official URL: http://dx.doi.org/10.1099/vir.0.82445-0
Human enteric adenoviruses propagate poorly in conventional human cell lines used to grow other adenovirus serotypes. As human enteric adenoviruses have a defect in counteracting the cellular interferon (IFN) response in cell culture, to aid in growth of the virus, a 293-based cell line defective in its ability to respond to IFN was constructed. This cell line (293-SV5/V) constitutively expresses V-protein of the paramyxovirus Simian virus 5, which degrades the signal transducer and activator of transcription 1 (STAT1) and thereby prevents the STAT1-mediated IFN response. Analysis of human enteric adenovirus type 40 (HAdV-40)-infected 293-SV5/V cells compared with parental 293 cells shows that the recombinant line allows more rapid production of virus and results in higher titres. These results suggest that the defect in HAdV-40 in counteracting the IFN response can be overcome at least partially through the use of 293-SV5/V cell lines.
|Item Type:||Journal Article|
|Subjects:||T Technology > TP Chemical technology
Q Science > QR Microbiology > QR355 Virology
|Divisions:||Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)|
|Journal or Publication Title:||Journal of General Virology|
|Publisher:||Society for General Microbiology|
|Number:||Number Part 1|
|Number of Pages:||6|
|Page Range:||pp. 71-76|
|Access rights to Published version:||Restricted or Subscription Access|
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