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Extension of a genetic network model by iterative experimentation and mathematical analysis
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Locke, James C. W., Southern, Megan M., Kozma-Bognar, Laszlo, Hibberd, V. (Victoria), Brown, Paul E., Turner, Matthew S. and Millar, A. J. (Andrew J.) (2005) Extension of a genetic network model by iterative experimentation and mathematical analysis. MOLECULAR SYSTEMS BIOLOGY . doi:10.1038/msb4100018 ISSN 1744-4292.
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Official URL: http://dx.doi.org/10.1038/msb4100018
Abstract
Circadian clocks involve feedback loops that generate rhythmic expression of key genes. Molecular genetic studies in the higher plant Arabidopsis thaliana have revealed a complex clock network. The first part of the network to be identified, a transcriptional feedback loop comprising TIMING OF CAB EXPRESSION 1 (TOC1), LATE ELONGATED HYPOCOTYL (LHY) and CIRCADIAN CLOCK ASSOCIATED 1 (CCA1), fails to account for significant experimental data. We develop an extended model that is based upon a wider range of data and accurately predicts additional experimental results. The model comprises interlocking feedback loops comparable to those identified experimentally in other circadian systems. We propose that each loop receives input signals from light, and that each loop includes a hypothetical component that had not been explicitly identified. Analysis of the model predicted the properties of these components, including an acute light induction at dawn that is rapidly repressed by LHY and CCA1. We found this unexpected regulation in RNA levels of the evening-expressed gene GIGANTEA (GI), supporting our proposed network and making GI a strong candidate for this component.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QD Chemistry | ||||
Journal or Publication Title: | MOLECULAR SYSTEMS BIOLOGY | ||||
Publisher: | NATURE PUBLISHING GROUP | ||||
ISSN: | 1744-4292 | ||||
Official Date: | 2005 | ||||
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Number of Pages: | 9 | ||||
DOI: | 10.1038/msb4100018 | ||||
Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
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