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Scanning electrochemical microscopy: principles and applications to biophysical systems
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Edwards, Martin A., Martin, Sophie, Whitworth, Anna L., Macpherson, Julie V. and Unwin, Patrick R. (2006) Scanning electrochemical microscopy: principles and applications to biophysical systems. PHYSIOLOGICAL MEASUREMENT, 27 (12). R63-R108. doi:10.1088/0967-3334/27/12/R01
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Official URL: http://dx.doi.org/10.1088/0967-3334/27/12/R01
Abstract
This review highlights numerous and wide ranging biophysical and biochemical applications of scanning electrochemical microscopy (SECM). SECM instrumentation and theoretical modelling, necessary for experimental interpretation, are outlined, followed by a detailed discussion of the diverse applications of this technique. These include the measurement of flow through membranes, the determination of kinetic parameters of reactions, the investigation of the permeability of small molecules in tissues and monitoring biological processes, such as the production of oxygen or nitric oxide by cells. The significant impact of micro-electrochemical techniques on our understanding of basic physicochemical processes at biologically relevant interfaces is also considered. Studies reviewed include transport across and within bilayers and monolayers. Recent advances in SECM include the combination of SECM with other techniques, such as atomic force microscopy and optical microscopy. These developments are highlighted, along with prospects for the future.
Item Type: | Journal Item | ||||
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Subjects: | Q Science > QH Natural history > QH301 Biology R Medicine Q Science > QP Physiology |
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Journal or Publication Title: | PHYSIOLOGICAL MEASUREMENT | ||||
Publisher: | IOP PUBLISHING LTD | ||||
ISSN: | 0967-3334 | ||||
Official Date: | December 2006 | ||||
Dates: |
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Volume: | 27 | ||||
Number: | 12 | ||||
Number of Pages: | 46 | ||||
Page Range: | R63-R108 | ||||
DOI: | 10.1088/0967-3334/27/12/R01 | ||||
Publication Status: | Published |
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