Naylor, Richard William, 1983-, Collins, Robert J., Philpott, Anna and Jones, E. A. (Elizabeth A.). (2009) Normal levels of p27Xic1 are necessary for somite segmentation and determining pronephric organ size. Organogenesis, Vol.5 (No.4). pp. 201-210. ISSN 1547-6278

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Abstract

The Xenopus laevis cyclin dependent kinase inhibitor p27Xic1 has been shown to be involved in exit from the cell cycle and differentiation of cells into a quiescent state in the nervous system, muscle tissue, heart and retina. We show that p27Xic1 is expressed in the developing kidney in the nephrostomal regions. Using over-expression and morpholino oligonucleotide (MO) knock-down approaches we show normal levels of p27Xic1 regulate pronephros organ size by regulating cell cycle exit. Knock-down of p27Xic1 expression using a MO prevented myogenesis, as previously reported; an effect that subsequently inhibits pronephrogenesis. Furthermore, we show that normal levels of p27Xic1 are required for somite segmentation also through its cell cycle control function. Finally, we provide evidence to suggest correct paraxial mesoderm segmentation is not necessary for pronephric induction in the intermediate mesoderm. These results indicate novel developmental roles for p27Xic1, and reveal its differentiation function is not universally utilised in all developing tissues.

Item Type:	Journal Article
Subjects:	Q Science > QL Zoology
Divisions:	Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010) Faculty of Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH):	Xenopus laevis, Kidneys -- Size, Kidneys -- Research, Somite, Cell cycle
Journal or Publication Title:	Organogenesis
Publisher:	Landes Bioscience
ISSN:	1547-6278
Date:	2009
Volume:	Vol.5
Number:	No.4
Page Range:	pp. 201-210
Identification Number:	10.4161/org.5.4.9973
Status:	Peer Reviewed
Access rights to Published version:	Open Access
Funder:	Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Wellcome Trust (London, England)
Grant number:	BB/ C00406X/1 (BBSRC)
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URI:	http://wrap.warwick.ac.uk/id/eprint/3280

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