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Type III polyketide synthase beta-ketoacyl-ACP starter unit and ethylmalonyl-CoA extender unit selectivity discovered by Streptomyces coelicolor genome mining
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Song, Lijiang, Barona-Gomez, Francisco, Corre, Christophe, Xiang, Longkuan, Udwary, Daniel W., Austin, Michael B., Noel, Joseph P., Moore, Bradley S. and Challis, Gregory L. (2006) Type III polyketide synthase beta-ketoacyl-ACP starter unit and ethylmalonyl-CoA extender unit selectivity discovered by Streptomyces coelicolor genome mining. Journal of the American Chemical Society, 128 (46). pp. 14754-14755. doi:10.1021/ja065247w ISSN 0002-7863.
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Official URL: http://dx.doi.org/10.1021/ja065247w
Abstract
Polyketide synthases (PKSs) are involved in the biosynthesis of many important natural products. In bacteria, type III PKSs typically catalyze iterative decarboxylation and condensation reactions of malonyl-CoA building blocks in the biosynthesis of polyhydroxyaromatic products. Here it is shown that Gcs, a type III PKS encoded by the sco7221 ORF of the bacterium Streptomyces coelicolor, is required for biosynthesis of the germicidin family of 3,6-dialkyl-4-hydroxypyran-2-one natural products. Evidence consistent with Gcs-catalyzed elongation of specific β-ketoacyl-ACP products of the fatty acid synthase FabH with ethyl- or methylmalonyl-CoA in the biosynthesis of germicidins is presented. Selectivity for β-ketoacyl-ACP starter units and ethylmalonyl-CoA as an extender unit is unprecedented for type III PKSs, suggesting these enzymes may be capable of utilizing a far wider range of starter and extender units for natural product assembly than believed until now.
| Item Type: | Journal Article | ||||||
|---|---|---|---|---|---|---|---|
| Subjects: | Q Science > QD Chemistry | ||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) |
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| Journal or Publication Title: | Journal of the American Chemical Society | ||||||
| Publisher: | American Chemical Society | ||||||
| ISSN: | 0002-7863 | ||||||
| Official Date: | 22 November 2006 | ||||||
| Dates: |
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| Volume: | 128 | ||||||
| Number: | 46 | ||||||
| Page Range: | pp. 14754-14755 | ||||||
| DOI: | 10.1021/ja065247w | ||||||
| Status: | Peer Reviewed | ||||||
| Publication Status: | Published | ||||||
| Access rights to Published version: | Restricted or Subscription Access |
Data sourced from Thomson Reuters' Web of Knowledge
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