The Library
Activation of the extracellular calcium-sensing receptor initiates insulin secretion from human islets of Langerhans: involvement of protein kinases
Tools
Tools
Tools
Gray, Elizabeth, Muller, Dany, Squires, Paul E., Asare-Anane, Henry, Huang, Guo-Cai, Amiel, Stephanie, Persaud, Shanta J. and Jones, Peter M.. (2006) Activation of the extracellular calcium-sensing receptor initiates insulin secretion from human islets of Langerhans: involvement of protein kinases. JOURNAL OF ENDOCRINOLOGY, 190 (3). pp. 703-710. ISSN 0022-0795
Full text not available from this repository.
Official URL: http://dx.doi.org/10.1677/joe.1.06891
Abstract
The extracellular calcium-sensing receptor (CaR) is usually associated with systemic Ca2+ homeostasis, but the CaR is also expressed in many other tissues, including pancreatic islets of Langerhans. In the present study, we have used human islets and an insulin-secreting cell line (MIN6) to investigate the effects of CaR activation using the calcimimetic R-568, a CaR agonist that activates the CaR it physiological concentrations of extracellular Ca2+. CaR activation initiated a marked but transient insulin secretory response from both human islets and MIN6 cells at a sub-stimulatory concentration of glucose, and further enhanced glucose-induced insulin secretion. CaR-induced insulin secretion was reduced by inhibitors of phospholipase C or calcium-calmodulin-dependent kinases, but not by a protein kinase C inhibitor. CaR activation was also associated with an activation of p42/44 mitogen-activated protein kinases (MAPK), and CaR-induced insulin secretion was reduced by an inhibitor of p42/44 MAPK activation. We suggest that the P-cell CaR is activated by divalent cations co-released with insulin, and that this may be an important mechanism of intra-islet communication between beta-cells.
| Item Type: | Journal Article |
|---|---|
| Subjects: | R Medicine > RC Internal medicine |
| Journal or Publication Title: | JOURNAL OF ENDOCRINOLOGY |
| Publisher: | SOC ENDOCRINOLOGY |
| ISSN: | 0022-0795 |
| Date: | September 2006 |
| Volume: | 190 |
| Number: | 3 |
| Number of Pages: | 8 |
| Page Range: | pp. 703-710 |
| Identification Number: | 10.1677/joe.1.06891 |
| Publication Status: | Published |
| URI: | http://wrap.warwick.ac.uk/id/eprint/32935 |
Data sourced from Thomson Reuters' Web of Knowledge
Actions (login required)
 |
View Item |
