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Identification of chemerin receptor (ChemR23) in human endothelial cells: chemerin-induced endothelial angiogenesis

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Kaur, Jaspreet, Adya, Raghu, Tan, Bee K., Chen, Jing and Randeva, Harpal S.. (2010) Identification of chemerin receptor (ChemR23) in human endothelial cells: chemerin-induced endothelial angiogenesis. Biochemical and Biophysical Research Communications, Vol.391 (No.4). pp. 1762-1768. ISSN 0006-291X

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Official URL: http://dx.doi.org/10.1016/j.bbrc.2009.12.150

Abstract

Chemerin acting via its distinct G protein-coupled receptor CMKLR1 (ChemR23), is a novel adipokine, circulating levels of which are raised in inflammatory states. Chemerin shows strong correlation with various facets of the metabolic syndrome; these states are associated with an increased incidence of cardiovascular disease (CVD) and dysregulated angiogenesis. We therefore, investigated the regulation of ChemR23 by pro-inflammatory cytokines and assessed the angiogenic potential of chemerin in human endothelial cells (EC). We have demonstrated the novel presence of ChemR23 in human ECs and its significant up-regulation (P < 0.001) by pro-inflammatory cytokines, TNF-α, IL-1β and IL-6. More importantly, chemerin was potently angiogenic, as assessed by conducting functional in-vitro angiogenic assays; chemerin also dose-dependently induced gelatinolytic (MMP-2 & MMP-9) activity of ECs (P < 0.001). Furthermore, chemerin dose-dependently activated PI3K/Akt and MAPKs pathways (P < 0.01), key angiogenic and cell survival cascades. Our data provide the first evidence of chemerin-induced endothelial angiogenesis and MMP production and activity.

Item Type:	Journal Article
Subjects:	R Medicine > RC Internal medicine
Divisions:	Faculty of Medicine > Warwick Medical School > Clinical Sciences Research Institute (CSRI) Faculty of Medicine > Warwick Medical School > Metabolic and Vascular Health Faculty of Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH):	Retinoids, Cytokines -- Research, Neovascularization -- Regulation, Vascular endothelial growth factors, Cardiovascular system -- Diseases
Journal or Publication Title:	Biochemical and Biophysical Research Communications
Publisher:	Elsevier
ISSN:	0006-291X
Date:	22 January 2010
Volume:	Vol.391
Number:	No.4
Page Range:	pp. 1762-1768
Identification Number:	10.1016/j.bbrc.2009.12.150
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	General Charities of the City of Coventry (GCCC)
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URI:	http://wrap.warwick.ac.uk/id/eprint/3300