Sweeney, F. P., Courtenay, Orin, Ul-Hassan, Atiya, Hibberd, V. (Victoria), Reilly, L. A. and Wellington, E. M. H. (Elizabeth M. H.), 1954-. (2006) Immunomagnetic recovery of Mycobacterium bovis from naturally infected environmental samples. LETTERS IN APPLIED MICROBIOLOGY, 43 (4). pp. 364-369. ISSN 0266-8254

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Abstract

Aims: To adapt an immunomagnetic capture (IMC) technique to concentrate and cultivate Mycobacterium bovis from environmental samples including soil, faeces and urine. Methods and Results: Cells of Myco. bovis BCG and wild-type Myco. bovis were successfully isolated and cultured from seeded and naturally infected materials respectively. The IMC cell recovery estimated by colony forming units (CFUs) counts ranged from 0.10% to 0.16% for spiked media, and 0.15-0.36% for naturally infected soil and faeces. Recovery estimated by cell counts calculated using semi-quantitative PCR ranged from 80.3% to 88.6% for spiked and 84.1-88.2% for naturally infected material. The differences in the recovery rates estimated by CFUs compared with pixel intensity is likely to be due to clustering of cells on culture plates, thereby underestimating the true cell count. Conclusions: The IMC techniques can be applied to isolate viable wild type Myco. bovis from naturally contaminated environmental samples. Significance and Impact of Study: Cultivation of Myco. bovis from environmental samples using traditional methods is extremely problematic. Here, we demonstrate a novel development of IMC techniques that will greatly facilitate the study of the organism in situ in order to assess its epidemiological importance in bovine tuberculosis persistence.

Item Type:	Journal Article
Subjects:	T Technology > TP Chemical technology Q Science > QR Microbiology
Journal or Publication Title:	LETTERS IN APPLIED MICROBIOLOGY
Publisher:	BLACKWELL PUBLISHING
ISSN:	0266-8254
Date:	October 2006
Volume:	43
Number:	4
Number of Pages:	6
Page Range:	pp. 364-369
Identification Number:	10.1111/j.1472-765X.2005.01983.x
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/33094

Data sourced from Thomson Reuters' Web of Knowledge

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