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Expression, purification and preliminary crystallographic analysis of oligopeptidase B from Trypanosoma brucei
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Rea, Dean, Hazell, Carole, Andrews, Norma W., Morty, Rory E. and Fülöp, Vilmos (2006) Expression, purification and preliminary crystallographic analysis of oligopeptidase B from Trypanosoma brucei. Acta Crystallographica Section F - Structural Biology and Crystallization Communications, Vol.62 (Part 8). pp. 808-810. doi:10.1107/S1744309106027874 ISSN 1744-3091.
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Official URL: http://dx.doi.org/10.1107/S1744309106027874
Abstract
African sleeping sickness, also called trypanosomiasis, is a significant cause of morbidity and mortality in sub-Saharan Africa. Peptidases from Trypanosoma brucei, the causative agent, include the serine peptidase oligopeptidase B, a documented virulence factor and therapeutic target. Determination of the three-dimensional structure of oligopeptidase B is desirable to facilitate the development of novel inhibitors. Oligopeptidase B was overexpressed in Escherichia coli as an N-terminally hexahistidine-tagged fusion protein, purified using metal-affinity chromatography and crystallized using the hanging-drop vapour-diffusion technique in 7%(w/v) polyethylene glycol 6000, 1 M LiCl, 0.1 M bis-tris propane pH 7.5. Diffraction data to 2.7 angstrom resolution were collected using synchrotron radiation. The crystals belong to space group P3(1)21 or P3(2)21, with unit-cell parameters a = b = 124.5, c = 249.9 angstrom. A complete data set to 2.7 angstrom was collected using synchrotron radiation.
| Item Type: | Journal Article | ||||
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| Subjects: | Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Biological Sciences ( -2010) Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) |
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| Journal or Publication Title: | Acta Crystallographica Section F - Structural Biology and Crystallization Communications | ||||
| Publisher: | Wiley-Blackwell Publishing, Inc. | ||||
| ISSN: | 1744-3091 | ||||
| Official Date: | August 2006 | ||||
| Dates: |
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| Volume: | Vol.62 | ||||
| Number: | Part 8 | ||||
| Number of Pages: | 3 | ||||
| Page Range: | pp. 808-810 | ||||
| DOI: | 10.1107/S1744309106027874 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Open Access (Creative Commons) |
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