The Library
Expression, purification and preliminary crystallographic analysis of oligopeptidase B from Trypanosoma brucei
Tools
Tools
Tools
Rea, Dean, Hazell, Carole, Andrews, Norma W., Morty, Rory E. and Fülöp, Vilmos. (2006) Expression, purification and preliminary crystallographic analysis of oligopeptidase B from Trypanosoma brucei. Acta Crystallographica Section F - Structural Biology and Crystallization Communications, Vol.62 (Part 8). pp. 808-810. ISSN 1744-3091
Full text not available from this repository.
Official URL: http://dx.doi.org/10.1107/S1744309106027874
Abstract
African sleeping sickness, also called trypanosomiasis, is a significant cause of morbidity and mortality in sub-Saharan Africa. Peptidases from Trypanosoma brucei, the causative agent, include the serine peptidase oligopeptidase B, a documented virulence factor and therapeutic target. Determination of the three-dimensional structure of oligopeptidase B is desirable to facilitate the development of novel inhibitors. Oligopeptidase B was overexpressed in Escherichia coli as an N-terminally hexahistidine-tagged fusion protein, purified using metal-affinity chromatography and crystallized using the hanging-drop vapour-diffusion technique in 7%(w/v) polyethylene glycol 6000, 1 M LiCl, 0.1 M bis-tris propane pH 7.5. Diffraction data to 2.7 angstrom resolution were collected using synchrotron radiation. The crystals belong to space group P3(1)21 or P3(2)21, with unit-cell parameters a = b = 124.5, c = 249.9 angstrom. A complete data set to 2.7 angstrom was collected using synchrotron radiation.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010) Faculty of Science > Life Sciences (2010- ) |
| Journal or Publication Title: | Acta Crystallographica Section F - Structural Biology and Crystallization Communications |
| Publisher: | Wiley-Blackwell Publishing, Inc. |
| ISSN: | 1744-3091 |
| Date: | August 2006 |
| Volume: | Vol.62 |
| Number: | Part 8 |
| Number of Pages: | 3 |
| Page Range: | pp. 808-810 |
| Identification Number: | 10.1107/S1744309106027874 |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Open Access |
| URI: | http://wrap.warwick.ac.uk/id/eprint/33269 |
Data sourced from Thomson Reuters' Web of Knowledge
Actions (login required)
 |
View Item |
